Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

71091 NovaTaq™ Hot Start DNA Polymerase

NovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase

NovaTaq™ Hot Start DNA Polymerase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
71091
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71091-3 Glass bottle 250 u
71091-4 Glass bottle 1250 u
Description
OverviewNovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.
Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration 5.0 U/µl
Endonuclease None detected
Exonuclease None detected
Amplification effiency Functional PCR
Storage –20°C
Catalogue Number71091 Brand Family Novagen®
Features and benefits
  • Higher PCR specificity and yield
  • Improved low-copy target amplification
  • Ambient temperature setup compatible with automation
  • Target amplification of up to 5 kbp
  • Ideal for quantitative and high-throughput PCR applications
References
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.
•250 U or 5 × 250 UNovaTaq Hot Start DNA Polymerase (5 U/µl)
•1.5 ml or 5 × 1.5 ml10X NovaTaq Hot Start Buffer
•1.5 ml or 5 × 1.5 ml25 mM MgCl₂
DeclarationPurchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
Quality LevelMQ100
Applications
ApplicationNovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71091-3 04055977271751
71091-4 04055977271768