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GF149 PDGF-BB, recombinant human

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

PDGF-BB, recombinant human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P01127, P31240 (mouse), Q05028 (rat)
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GF149
10 µg  
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Overview

Replacement Information

Key Specifications Table

Key ApplicationsSpeciesSourcePurity
CULTHuman E. coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
Description
Catalogue NumberGF149
ReplacesGF018
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionPDGF-BB, recombinant human
Alternate Names
  • Platelet Derived Growth Factor-BB
Background InformationPlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
References
Product Information
Formulation
PresentationSterile filtered solution lyophilized from 10 mM HOAc.
Quality LevelMQ100
Applications
ApplicationThe Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
Key Applications
  • Cell Culture
Application NotesFor most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.
Biological Information
PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
SourceE. coli
Specific ActivityHuman PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.
Gene Symbol
  • PDGFB
  • PDGF2
  • SIS
UniProt Number
Stem Cell Type
  • Neural Stem Cells
  • Mesenchymal Stem Cells
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GF149 04053252275746

Documentation

PDGF-BB, recombinant human SDS

Title

Safety Data Sheet (SDS) 

PDGF-BB, recombinant human Certificates of Analysis

TitleLot Number
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 1997734 1997734
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 2120320 2120320
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 2250502 2250502
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 2975576 2975576
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3307477 3307477
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3448673 3448673
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3683899 3683899
PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3884316 3884316
PDGF-BB, HUMAN RECOMBINANT PROTEIN - DAM1593819 DAM1593819
PDGF-BB, HUMAN RECOMBINANT PROTEIN - DAM1770456 DAM1770456
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Data Sheet

Title
PDGF-BB, HUMAN RECOMBINANT PROTEIN

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