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14-791M PI 3-Kinase (p110α(E542K)/p85α) murine, 250 µg

Complex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K & untagged, recombinant, murine p85α full length.

14-791M
250 µg  Also available in 10 μg size (cat#14-791) and in bulk (cat# 14-791-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-791M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI 3-Kinase (p110α(E542K)/p85α) murine, 250 µg
OverviewComplex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length.
Background InformationThe E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
References
Product Information
Presentation50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ300
Applications
ApplicationComplex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K & untagged, recombinant, murine p85α full length.
Biological Information
SourceCo-expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • PIK3CA
  • PI3K
  • PK3CA
Protein TargetPI3Kα/p85α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPI3K/PI4K
UniProt Number
Molecular Weightp110α(E542K) MW = 129 kDa, p85α MW = 84 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-791) and in bulk (cat# 14-791-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-791M 04053252322556

Documentation

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-791 PI 3-Kinase (p110α(E542K)/p85α) murine, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases