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14-786M PI3 Kinase (p110α/p65α) Murine, 250 µg

Complex of N-terminal 6His-tagged, recombinant, full-length mouse p110α & untagged, recombinant, full length mouse p65α. For use in Kinase Assays.

PI3 Kinase (p110α/p65α) Murine, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-786M
250 µg  Also available in 10 μg size (cat#14-786) and in bulk (cat# 14-786-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-786M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI3 Kinase (p110α/p65α) Murine, 250 µg
OverviewComplex of N-terminal 6His-tagged, recombinant, full-length mouse p110α and untagged, recombinant, full length mouse p65α.
Background Informationp65α is an oncogenic mutant form of p85α(residues 1-571) which leads to increased PI3K activity.
References
Product Information
Quality LevelMQ300
Applications
ApplicationComplex of N-terminal 6His-tagged, recombinant, full-length mouse p110α & untagged, recombinant, full length mouse p65α. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceCoexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • PIK3CA
  • PI3K
  • MGC142161
  • p110-alpha
  • MGC142163
Protein TargetPI3Kα/p65α
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Molecular Weightp110α MW = 129kDa, p65α MW = 65.9kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-786) and in bulk (cat# 14-786-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-786M 04053252333132

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-786 PI3 Kinase (p110α/p65α) Murine, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases