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14-558-D PI3 kinase (p120Ɣ)

Recombinant human p120Ɣ, full length, with N-terminal His-tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

PI3 kinase (p120Ɣ) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-558-D
20 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-558-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI3 kinase (p120Ɣ)
OverviewRecombinant human p120Ɣ, full length, with N-terminal His-tag,
References
Product Information
Presentation25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol
Quality LevelMQ300
Applications
ApplicationRecombinant human p120Ɣ, full length, with N-terminal His-tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration0.22 mg/ml
Purity≥80%
SourceSf9 insect cells
Specific Activity1226 pmol/min/μg.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
Gene Symbol
  • PIK3CG
  • p110-gamma
  • PI3-kinase
  • PIK3
  • PI3K
  • PI3Kgamma
  • PTDINS-3-kinase
  • PI3CG
  • p120-PI3K
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P48736 # 3-phosphorylates the cellular phosphoinositide PtdIns- 4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5- triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
SIZE: 1102 amino acids; 126454 Da
SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit.
TISSUE SPECIFICITY: Pancreas, skeletal muscle, liver and heart.
SIMILARITY: SwissProt: P48736 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
Molecular Weight130 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: 45 min followed by incubation with the Kinase Detection reagent (50 μL) for 55 min.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size20 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-558-D 04055977276619