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539576 PKA, Catalytic Subunit, Bovine Heart

PKA, Catalytic Subunit, Bovine Heart MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase A, Catalytic Subunit, cAMP-Dependent Protein Kinase Catalytic Subunit

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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
539576-25UG Glass bottle 25 μg
Description
OverviewNative PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
Note: 25 µg ≥ 500,000 units.
Catalogue Number539576
Brand Family Calbiochem®
SynonymsProtein Kinase A, Catalytic Subunit, cAMP-Dependent Protein Kinase Catalytic Subunit
Application Data
References
ReferencesCorbin, J.D., et al. 1988. Methods Enzymol 159, 74.
Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
FormLiquid
FormulationIn 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥20,000 units/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
539576-25UG 04055977194739

Documentation

PKA, Catalytic Subunit, Bovine Heart SDS

Title

Safety Data Sheet (SDS) 

PKA, Catalytic Subunit, Bovine Heart Certificates of Analysis

TitleLot Number
539576

References

Reference overview
Corbin, J.D., et al. 1988. Methods Enzymol 159, 74.
Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-May-2017 JSW
SynonymsProtein Kinase A, Catalytic Subunit, cAMP-Dependent Protein Kinase Catalytic Subunit
Application Data
DescriptionNative PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
FormLiquid
FormulationIn 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Kinase Activity Assay: The activity of the PKA catalytic subunit is assayed in 40 mM Tris-HCl, 20 mM magnesium acetate, 200 µM [γ-32P]ATP (500-1000 cpm/pmol) with 130 µM Kemptide substrate in a total volume of 60 µl. The reaction is incubated at 30°C for 5 min and terminated by spotting 40 µl of the reaction mix onto P-81 filters and washing in 0.5% H3PO4 for 5 min for a total of 4 washes. Following the final wash, the filters are rinsed in ethanol, dried, and counted in the presence of scintillation fluid. A value of 200 pmol obtained for Kemptide phosphorylation under these conditions corresponds to 1 pmol phosphorylated casein.
Purity≥90% by SDS-PAGE
Specific activity≥20,000 units/µg protein
Unit definitionOne unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesCorbin, J.D., et al. 1988. Methods Enzymol 159, 74.
Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.