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539610 PKCζ/ι Pseudosubstrate Inhibitor - Calbiochem

The PKCζ/ι Pseudosubstrate Inhibitor controls the biological activity of PKCζ/ι. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: SIYRRGARRWRKL, Protein Kinase Cζ/ι Pseudosubstrate Inhibitor

Primary Target: PKCζ
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539610
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₇₆H₁₂₈N₃₀O₁₆

Products

Catalog NumberPackaging Qty/Pack
539610-500UG Plastic ampoule 500 μg
Description
OverviewA selective, reversible, and substrate competitive inhibitor of protein kinase Cζ and protein kinase Cι (PKCζ/ι) isozymes.
Catalogue Number539610
Brand Family Calbiochem®
SynonymsSIYRRGARRWRKL, Protein Kinase Cζ/ι Pseudosubstrate Inhibitor
References
ReferencesSajan, M.P., et al. 1999. J. Biol. Chem. 274, 30495.
Laudanna, C., et al. 1998. J. Biol. Chem. 273, 30306.
Standaert, M.L., et al. 1997. J. Biol. Chem. 272, 30075.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₇₆H₁₂₈N₃₀O₁₆
Chemical formulaC₇₆H₁₂₈N₃₀O₁₆
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetPKCζ
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceH-Ser-Ile-Tyr-Arg-Arg-Gly-Ala-Arg-Arg-Trp-Arg-Lys-Leu-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
539610-500UG 04055977194777

Documentation

PKCζ/ι Pseudosubstrate Inhibitor - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

PKCζ/ι Pseudosubstrate Inhibitor - Calbiochem Certificates of Analysis

TitleLot Number
539610

References

Reference overview
Sajan, M.P., et al. 1999. J. Biol. Chem. 274, 30495.
Laudanna, C., et al. 1998. J. Biol. Chem. 273, 30306.
Standaert, M.L., et al. 1997. J. Biol. Chem. 272, 30075.

Brochure

Title
PKC Pathway Poster PDF ( 676 KB )
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-January-2010 RFH
SynonymsSIYRRGARRWRKL, Protein Kinase Cζ/ι Pseudosubstrate Inhibitor
DescriptionNon-cell-permeable protein kinase Cζ and protein kinase Cι pseudosubstrate sequence peptide that can be used as a selective, reversible, and substrate competitive inhibitor PKCζ/ι in in vitro kinase assays.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Recommended reaction conditions
PKCζ or PKCι -containing sample is incubated at 30-37°C, with or without 400 µM PKCζ/ι pseudosubstrate inhibitor, in 50 mM Tris-HCl buffer (pH 7.5) containing 5 mM MgCl2, 100 µM Na3VO4, 100 µM Na4P2O7, 1 mM NaF, 100 µM PMSF, 50 µM ATP, 5 µCi [γ-32P]ATP, 4 µg phosphatidylserine, and 40 µM PKCζ/ι substrate (Cat. No. 539667).
Chemical formulaC₇₆H₁₂₈N₃₀O₁₆
Peptide SequenceH-Ser-Ile-Tyr-Arg-Arg-Gly-Ala-Arg-Arg-Trp-Arg-Lys-Leu-OH
Purity≥95% by HPLC
SolubilityGlacial acetic acid (2 mg/ml) or H₂O (2 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesSajan, M.P., et al. 1999. J. Biol. Chem. 274, 30495.
Laudanna, C., et al. 1998. J. Biol. Chem. 273, 30306.
Standaert, M.L., et al. 1997. J. Biol. Chem. 272, 30075.