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109254 Papanicolaou's solution 1b Hematoxylin solution S

for cytology

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109254
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
1092540500 Plastic bottle 500 ml
1092542500 Plastic bottle 2.5 l
Description
Catalogue Number109254
DescriptionPapanicolaou’s solution 1b Hematoxylin solution S
OverviewPapanicolaou’s solution 1b Hematoxylin solution S - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat no.109254) give a blue to dark violet staining result with clinical specimen material.
A 500 ml bottle of either solution is sufficient for 1500 -2500 slides. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor cytology
Biological Information
Physicochemical Information
Density1.05 g/cm3 (20 °C)
pH value2.5 (H₂O, 20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H302: Harmful if swallowed.
H319: Causes serious eye irritation.
H373: May cause damage to organs through prolonged or repeated exposure if swallowed.
Precautionary Statement(s)P260: Do not breathe mist or vapours.
P264: Wash skin thoroughly after handling.
P280: Wear eye protection/ face protection.
P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P314: Get medical advice/ attention if you feel unwell.
Signal WordWarning
Storage class10 - 13 Other liquids and solids
WGKWGK 1 slightly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerharmful
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)passes test
Nucleiblue to dark violet
Cyanophilic Cytoplasm (basophilic)blue-green
Eosinophilic Cytoplasm (acidophilic)pink
Global Trade Item Number
Catalog Number GTIN
1092540500 04022536112510
1092542500 04022536112534

Related Products & Applications

Accessories (Primary)

Catalog Number Description
109270 Papanicolaou's solution 3c Polychromatic solution EA 65
106887 Papanicolaou’s solution 2b Orange II solution
106888 Papanicolaou's solution 2a Orange G solution (OG 6)
109269 Papanicolaou's solution 3d polychromatic solution EA 65
109271 Papanicolaou's solution 3a polychromatic solution EA 31
115938 Hematoxylin monohydrate (C.I.75290)

Accessories (Secondary)

Catalog Number Description
109270 Papanicolaou's solution 3c Polychromatic solution EA 65
104302 Hematoxylin cryst. (C.I. 75290)
105175 Hematoxylin solution modified acc. to Gill II
106887 Papanicolaou’s solution 2b Orange II solution
106888 Papanicolaou's solution 2a Orange G solution (OG 6)
109269 Papanicolaou's solution 3d polychromatic solution EA 65
109271 Papanicolaou's solution 3a polychromatic solution EA 31

Related Products By: Application Facete

Clinical Cytology
Cytodiagnosis
Papanicolaou

Categories

IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents