69078 MilliporePerfect Protein™ HRP Western Blot Kit
Recommended Products
Overview
| Replacement Information |
|---|
Products
| Catalog Number | Packaging | Qty/Pack | |
|---|---|---|---|
| 69078-3 | Plastic ampoule | 25 blot |
| Description | |
|---|---|
| Overview | The Perfect Protein™ HRP Western Blot Kit includes an HRP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits. A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract. B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein. |
| Catalogue Number | 69078 |
| Brand Family | Novagen® |
| Features and benefits | • Detect markers simply by adding the S-protein
(AP or HRP Conjugate) or His•Tag® Monoclonal
Antibody to the same incubation used for
sample detection. • S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection. • Can be used with colorimetric and chemiluminescent AP or HRP substrates. • Recombinant, unmodified markers give sharp, accurately sized bands. • Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection. • Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa. |
| References |
|---|
| Product Information | |
|---|---|
| Components | |
| Form | Liquid |
| Applications |
|---|
| Biological Information |
|---|
| Physicochemical Information |
|---|
| Dimensions |
|---|
| Materials Information |
|---|
| Toxicological Information |
|---|
| Safety Information according to GHS |
|---|
| Safety Information |
|---|
| Product Usage Statements |
|---|
| Storage and Shipping Information | |
|---|---|
| Ship Code | Blue Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | -20°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Packaging Information |
|---|
| Transport Information |
|---|
| Supplemental Information |
|---|
| Specifications |
|---|
| Global Trade Item Number | |
|---|---|
| Catalog Number | GTIN |
| 69078-3 | 04055977259551 |
Documentation
Perfect Protein™ HRP Western Blot Kit SDS
| Title |
|---|
Perfect Protein™ HRP Western Blot Kit Certificates of Analysis
| Title | Lot Number |
|---|---|
| 69078 |
Brochure
| Title |
|---|
| Protein Purification and Detection Tools |
Citations
| Title | |
|---|---|
|
|
User Protocols
| Title |
|---|
| TB102 Perfect Protein™ Western Blot Kits |
| TB136 S-protein HRP Conjugate |