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516560 Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem

This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Islet-activating Protein

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516560
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Overview

Replacement Information

Key Specifications Table

CAS #
70323-44-3

Products

Catalog NumberPackaging Qty/Pack
516560-50UG 50 μg
Description
OverviewObtain a uniform suspension before use. A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt. Used in the study of adenylate cyclase regulation and the role of Gi proteins. Holotoxin activity is determined in a CHO cell assay.
NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
Catalogue Number516560
Brand Family Calbiochem®
SynonymsIslet-activating Protein
References
ReferencesJoshi, S.A., et al. 1998. FEBS Lett. 441, 67.
Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
Product Information
CAS number70323-44-3
ATP CompetitiveN
FormLyophilized
FormulationLyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetGuanine nucleotide-binding regulatory proteins Gi, Go, and Gt
PurityFive distinct bands by SDS-PAGE
Physicochemical Information
Cell permeableN
ContaminantsAdenylate cyclase activity: ≤35 pmol/min/µg in the presence of calmodulin
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXW5883750
Safety Information
R PhraseR: 23/24/25

Toxic by inhalation, in contact with skin and if swallowed.
S PhraseS: 36/37/39-45

Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
516560-50UG 04055977198157

Documentation

Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem Certificates of Analysis

TitleLot Number
516560

References

Reference overview
Joshi, S.A., et al. 1998. FEBS Lett. 441, 67.
Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

Citations

Title
  • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
  • Olga Pervushina, et al. (2004) Platelet factor 4/CXCL4 induces phagocytosis and the generation of reactive oxygen metabolites in mononuclear phagocytes independently of Gi protein activation or intracellular calcium transients. Journal of Immunology 173, 2060-2067.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision28-September-2007 RFH
    SynonymsIslet-activating Protein
    DescriptionA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and GT. Affects glucose homeostasis, immune cell function, and the induction of leukocytosis. Used in the study of adenylate cyclase regulation and the role of Gi proteins. Holotoxin activity is determined in CHO cell assay. Note: this product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, activation may be achieved by the methods of either Kaslow, H.R., and Burns, D.L. 1992. and Moss, J. 1983.
    FormLyophilized
    FormulationLyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.
    CAS number70323-44-3
    RTECSXW5883750
    PurityFive distinct bands by SDS-PAGE
    ContaminantsAdenylate cyclase activity: ≤35 pmol/min/µg in the presence of calmodulin
    SolubilityReconstitute with 500 µl H₂O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Special InstructionsFollowing reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
    Toxicity Toxic
    ReferencesJoshi, S.A., et al. 1998. FEBS Lett. 441, 67.
    Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
    Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
    Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
    Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
    Kaslow, H.R., and Burns, D.L., 1992. FASEB J. 6, 2684.
    Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
    Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
    Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
    Citation
  • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
  • Olga Pervushina, et al. (2004) Platelet factor 4/CXCL4 induces phagocytosis and the generation of reactive oxygen metabolites in mononuclear phagocytes independently of Gi protein activation or intracellular calcium transients. Journal of Immunology 173, 2060-2067.
    		•