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516561 Pertussis Toxin, Bordetella pertussis, Glycerol Solution - CAS 70323-44-3 - Calbiochem

Pertussis Toxin, Bordetella pertussis, Glycerol Solution, CAS 70323-44-3, is a protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Islet-activating Protein

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Overview

Replacement Information

Key Specifications Table

CAS #
70323-44-3

Products

Catalog NumberPackaging Qty/Pack
516561-50UG Alu drum 50 μg
Description
OverviewA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1, S-1 (A protomer) is responsible for the enzymatic activity of the toxin, and S-2, S-3, S-4, and S-5 form the B oligomer which is responsible for the toxins binding to the cell surface. NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
Catalogue Number516561
Brand Family Calbiochem®
SynonymsIslet-activating Protein
References
ReferencesFryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479
Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
Product Information
CAS number70323-44-3
ATP CompetitiveN
FormLiquid
FormulationIn 500 mM NaCl, 50 mM Tris, 10 mM glycine, 50% glycerol, pH 7.5.
ReversibleN
Quality LevelMQ200
Applications
Biological Information
Biological activity≤1 ng/ml as determined in a clustering assay using CHO cells line
Primary TargetGuanine nucleotide-binding regulatory proteins
Primary Target IC<sub>50</sub>≤1 ng/ml as determined in a clustering assay using CHO cells line
PurityFive distinct bands by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeableN
ContaminantsAdenylate cyclase.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXW5883750
Safety Information
R PhraseR: 23/24/25

Toxic by inhalation, in contact with skin and if swallowed.
S PhraseS: 36/37/39-45

Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsA uniform suspension may be achieved by gentle mixing prior to use.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
516561-50UG 04055977198171

Documentation

Pertussis Toxin, Bordetella pertussis, Glycerol Solution - CAS 70323-44-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Pertussis Toxin, Bordetella pertussis, Glycerol Solution - CAS 70323-44-3 - Calbiochem Certificates of Analysis

TitleLot Number
516561

References

Reference overview
Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479
Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-August-2023 JSW
SynonymsIslet-activating Protein
DescriptionA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. Used in the study of adenylate cyclase regulation and the role of Gi proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1. S-1 (a protomer) is responsible for the enzymatic activity of the toxin, and the other subunits (2-5) form the B oligomer which is responsible for the toxin's binding to the cell surface. Note: This product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, product may be activated by the methods of either Kaslow and Burns or Moss at al.(see references).
FormLiquid
FormulationIn 500 mM NaCl, 50 mM Tris, 10 mM glycine, 50% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number70323-44-3
RTECSXW5883750
PurityFive distinct bands by SDS-PAGE
ContaminantsAdenylate cyclase.
Biological activity≤1 ng/ml as determined in a clustering assay using CHO cells line
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsA uniform suspension may be achieved by gentle mixing prior to use.
Toxicity Toxic
ReferencesFryer, M.W. 1992. Neurosci. Lett. 146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479
Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.