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ST1013 PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb

This PhosphoDetect™ Anti-GSK-3α/β Rabbit pAb is validated for use in Dot Blot, ELISA, WB, ICC for the detection of GSK-3α/β.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)

ST1013
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
ST1013-10T Plastic ampoule 10 t
Description
OverviewRecognizes the ~51 kDa GSK-3α protein phosphorylated at Tyr279 and the ~47 kDa protein GSK-3β phosphorylated at Tyr216.
Note: 1T = 1 test. Sufficient for 10 Western miniblots.
Catalogue NumberST1013
Brand Family Calbiochem®
SynonymsAnti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)
Application Data
Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
References
ReferencesPapasozomenos, S.C., et al. 2002. PNAS 99, 1140.
Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Negative controlNormal rabbit serum
Positive controlSerum starved insulin-treated 3T3-L1 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Dot Blot
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesDot Blot (see comments)
ELISA (see coments)
Immunoblotting (1:1000)
Immunocytochemistry (1:400)
Application CommentsTo generate a positive control, serum starve 3T3-L1 cells for 3 h, then stimulate with 100 nM insulin or 100 ng/ml IGF-1 for 5 min. This antibody has also been reported to work for ELISA and dot blots. At a 1:1000 dilution there is enough antibody for 10 immunoblots at 10 ml/blot. Antibody must be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ST1013-10T 04055977208665

Documentation

PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb Certificates of Analysis

TitleLot Number
ST1013

References

Reference overview
Papasozomenos, S.C., et al. 2002. PNAS 99, 1140.
Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-May-2008 JSW
SynonymsAnti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)
ApplicationDot Blot (see comments)
ELISA (see coments)
Immunoblotting (1:1000)
Immunocytochemistry (1:400)
Application Data
Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~51 kDa phosphorylated GSK-3α protein phosphorylated at Ser279 and the ~47 kDa GSK-3β protein phosphorylated at Ser216.
BackgroundGSK-3α contains a phosphate on tyrosine 279, and GSK-3β contains a phosphate on tyrosine 216. GSK is a protein serine kinase involved in the control of regulatory proteins like glycogen synthase and the transcription factor c-Jun. It has also been associated with the regulation of the microtubule-binding protein Tau, and may be involved in the pathogenesis of Alzheimer's Disease.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
IsotypeIgG
Specieshuman, mouse, rat
Positive controlSerum starved insulin-treated 3T3-L1 cells
Negative controlNormal rabbit serum
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Preservative≤0.1% sodium azide
CommentsTo generate a positive control, serum starve 3T3-L1 cells for 3 h, then stimulate with 100 nM insulin or 100 ng/ml IGF-1 for 5 min. This antibody has also been reported to work for ELISA and dot blots. At a 1:1000 dilution there is enough antibody for 10 immunoblots at 10 ml/blot. Antibody must be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesPapasozomenos, S.C., et al. 2002. PNAS 99, 1140.
Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.