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407707 PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

This PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr1162/1163).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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407707
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
407707-10T Plastic ampoule 10 t
Description
OverviewRecognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
Catalogue Number407707
Brand Family Calbiochem®
Application Data
Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
References
ReferencesBevan, P. 2001. J. Cell Sci. 114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
Schmid, E., at al. 1998. FASEB J. 12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ebina, Y., et al. 1985. Cell 40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Application CommentsThe immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
407707-10T 04055977211474

Documentation

PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb Certificates of Analysis

TitleLot Number
407707

References

Reference overview
Bevan, P. 2001. J. Cell Sci. 114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
Schmid, E., at al. 1998. FASEB J. 12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ebina, Y., et al. 1985. Cell 40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-December-2007 JSW
ApplicationELISA (see comments)
Immunoblotting (1:1000)
Immunocytochemistry (see comments)
Application Data
Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr1162/1163.
BackgroundInsulin initiates its effects on cells through interaction with high-affinity cell surface glycoprotein receptors consisting of two α (~135 kDa) and two β (~95 kDa) subunits linked by disulfide bonds. The α subunits bind insulin and the β subunits become phosphorylated in response to insulin. Insulin-dependent phosphorylation occurs at multiple sites near the carboxyl terminus and in the kinase domain. The receptor protein tyrosine kinase has been implicated as the mediator of most, if not all, of the effects of insulin. Mutations that diminish both the phosphorylation and the internalization of the insulin receptor block insulin action, as do antibodies which inhibit protein tyrosine kinase activity. Cells from patients with diabetic syndromes, characterized by non-responsiveness to insulin, exhibit reduced insulin dependent protein tyrosine kinase activity. Phosphorylation at Tyr 1162, Tyr 1163, and Tyr 1158 is required for the tyrosine kinase activity of the insulin receptor.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor
IsotypeIgG
Specieshuman
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBevan, P. 2001. J. Cell Sci. 114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
Schmid, E., at al. 1998. FASEB J. 12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ebina, Y., et al. 1985. Cell 40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.