Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

525320 PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
525320
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
525320-100UG Plastic ampoule 100 μg
Description
OverviewPeroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Catalogue Number525320
Brand Family Calbiochem®
References
ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
Preservative0.1% Proclin
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (1:2000)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
Application CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
ClonePY20
HostMouse
IsotypeIgG2b
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
525320-100UG 07790788051129

Documentation

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate Certificates of Analysis

TitleLot Number
525320

References

Reference overview
Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-December-2007 JSW
ApplicationELISA (1:2000)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
DescriptionPurified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
HostMouse
ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
ClonePY20
IsotypeIgG2b
Speciesa broad range of species
FormLiquid
FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative0.1% Proclin
CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.