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PS1089 PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb

This PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PS1089
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
PS1089-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
Catalogue NumberPS1089
Brand Family Calbiochem®
Application Data
Detection of human RhoA, phosphorylated at Ser188, by immunoblotting. Samples: RhoA GST fusion protein left unphosphorylated (lanes 1,3) or phosphorylated in vitro by PKA (lanes 2,4,5,6). Primary antibodies: Anti-RhoA (detecting total Rho; lanes 1,2) and PhosphoDetect™ Anti-RhoA (pSer188) (Cat. No. PS1089) (1:1000) preincubated without blocking peptide (lanes 3,4), preincubated with phosphoRhoA (pSer188) blocking peptide (lane 5), and preincubated with nonspecific phosphoserine containing blocking peptide (lane 6). Detection: chemiluminescence.
References
ReferencesEllerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
Lang, P., et al. 1996. EMBO J. 15, 510.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
Positive controlRhoA GST fusion protein
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
ELISA (1:2000)
Application CommentsDoes not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PS1089-100UL 04055977226577

Documentation

PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb Certificates of Analysis

TitleLot Number
PS1089

References

Reference overview
Ellerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
Lang, P., et al. 1996. EMBO J. 15, 510.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-July-2008 JSW
ApplicationImmunoblotting (1:1000)
ELISA (1:2000)
Application Data
Detection of human RhoA, phosphorylated at Ser188, by immunoblotting. Samples: RhoA GST fusion protein left unphosphorylated (lanes 1,3) or phosphorylated in vitro by PKA (lanes 2,4,5,6). Primary antibodies: Anti-RhoA (detecting total Rho; lanes 1,2) and PhosphoDetect™ Anti-RhoA (pSer188) (Cat. No. PS1089) (1:1000) preincubated without blocking peptide (lanes 3,4), preincubated with phosphoRhoA (pSer188) blocking peptide (lane 5), and preincubated with nonspecific phosphoserine containing blocking peptide (lane 6). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.
BackgroundRho (A, B, and C) proteins are members of the Ras superfamily of GTPases. They regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and the inactive GDP-bound form. Conversion from the inactive to the active form and vice versa is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The three Rho proteins are highly homologous and contain the consensus sequences necessary for GDP/GTP binding and GTPase activity. RhoA is phosphorylated at Ser188 in vitro by PKA and PKG. This phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation, indicating that Ser188 is an important site for negative regulation of RhoA activity.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
IsotypeIgG
Specieshuman
Positive controlRhoA GST fusion protein
FormLiquid
FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDo not aliquot.
Toxicity Standard Handling
ReferencesEllerbroek, S.M., et al. 2003. J. Biol. Chem. 278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem. 273, 22554.
Lang, P., et al. 1996. EMBO J. 15, 510.