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506119 PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

This PhosphoDetect™ Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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506119
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ca, H, M, RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
506119-10T Plastic ampoule 10 t
Description
OverviewRecognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number506119
Brand Family Calbiochem®
Application Data
Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
References
ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
Nakagami, H., et al. 2001. Diabetes 50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
Han, J., et al. 1994. Science 265, 808.
Lee, J.C., et al. 1994. Nature 372, 739.
Rouse, J., et al. 1994. Cell 78, 1027.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1:1000)
Immunocytochemistry (see comments)
Application CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Canine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
506119-10T 04055977199451

Documentation

PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb Certificates of Analysis

TitleLot Number
506119

References

Reference overview
Marinissen, M.J., et al. 2001. Genes Dev. 15, 535.
Nakagami, H., et al. 2001. Diabetes 50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
Han, J., et al. 1994. Science 265, 808.
Lee, J.C., et al. 1994. Nature 372, 739.
Rouse, J., et al. 1994. Cell 78, 1027.

Brochure

Title
MAPK Pathway Poster ( 750 KB )
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2007 RFH
ApplicationImmunoblotting (1:1000)
Immunocytochemistry (see comments)
Application Data
Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
Backgroundp38 MAP kinase (MAPK), also called RK and CSBP, is the mammalian homologue of yeast HOG kinase and participates in a cascade that controls cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light, and growth factors. Both MKK3 and SEK phosphorylate p38 on tyrosine and threonine resulting in p38 activation. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
IsotypeIgG
Speciescanine, human, mouse, rat
Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Preservative≤0.1% sodium azide
CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
Nakagami, H., et al. 2001. Diabetes 50, 1472.
Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
Han, J., et al. 1994. Science 265, 808.
Lee, J.C., et al. 1994. Nature 372, 739.
Rouse, J., et al. 1994. Cell 78, 1027.