Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

71296 PhosphoSafe™ Extraction Reagent

Novagen's PhosphoSafe™ Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

PhosphoSafe™ Extraction Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
71296
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71296-3 Plastic ampoule 25 ml
71296-4 Glass bottle 125 ml
Description
OverviewPhosphoSafe™ Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster™ Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).


Catalogue Number71296
Brand Family Novagen®
Features and benefits
  • Phosphorylation state preserved during extraction
  • Compatible with kinase assays
References
ReferencesTrim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Toxic
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71296-3 07790788054229
71296-4 04055977254006

Documentation

PhosphoSafe™ Extraction Reagent SDS

Title

Safety Data Sheet (SDS) 

PhosphoSafe™ Extraction Reagent Certificates of Analysis

TitleLot Number
71296

References

Reference overview
Trim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.

Technical Info

Title
Western Blotting Quick Reference Product Guide

Citations

Title
  • Yanxia Liu, et al. (2007) A genomic screen for activators of the antioxidant response element. Procedings of the National Academy of Science 104, 5205-5210.
  • Elena Martinelli, et al. (2007) HIV-1 gp120 inhibits TLR9-mediated activation and IFN-α secretion in plasmacytoid dendritic cells. Procedings of the National Academy of Science 104, 3396-3401.
  • Dagmar Pommereit and Fred S. Wouters. (2007) An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth. Journal of Cell Science 120, 2694-2705.
  • Aicha Saadane, et al. (2007) Parthenolide inhibits IκB kinase, NF-κB activation, and inflammatory response in cystic fibrosis cells and mice. American Journal of Respiratory Cell and Molecular Biology 36, 728-736.
  • Jeremy O. Jones and Ann M. Arvin. (2006) Inhibition of the NF-κB pathway by Varicella-Zoster virus in vitro and in human epidermal cells in vivo. Journal of Virology 80, 5113-5124.
  • Jeremy O. Jones and Ann M. Arvin. (2006) Inhibition of the NF-κB pathway by varicella-zoster virus in vitro and in human epidermal cells in vivo. Journal of Virology 80, 5113-5124.
  • Marisol Quintero, et al. (2006) Mitochondria as signaling organelles in the vascular endothelium. Proceedings of the National Academy of Sciences (USA) 103, 5379-5384.
  • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
  • Anthony R. White, et al. (2006) Degradation of the alzheimer disease amyloid -peptide by metal-dependent up-regulation of metalloprotease activity. journal of Biological Chemistry 281, 17670-17680.
  • Ralf Gutzmer, et al. (2005) Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte-derived dendritic cells. Journal of Immunology 5224-5232.
  • Mauro Cesar Isoldi, et al. (2005) Rhabdomeric phototransduction initiated by the vertebrate photopigment melanopsin. Procedings of the National Academy of Science 102, 1217-1221.
  • Elad Katz, et al. (2005) MMTV Env encodes an ITAM responsible for transformation of mammary epithelial cells in three-dimensional culture. Journal of Experimental Medicine 201, 431-439.
  • Hiroki Kuroda, et al. (2005) Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation. Genes and Development 19, 1022-1027.
  • Markus Utech, et al. (2005) Mechanism of IFN-γ-Induced Endocytosis of Tight Junction Proteins: Mysoin II-Dependent Vacuolarization of the Apical Plasma Membrane. Molecular Biology of the Cell 16, 5040-5052.
    		•

    User Protocols

    Title
    TB402 PhosphoSafe™ Extraction Reagent