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17-499 Phosphorylation Explorer Pack Sample Kit

This Antibody pack contains Anti-phosphotyrosine Antibody, Anti-Phosphoserine Antibody, validated for use in WB, IF, Flow, IHC, ELISA, ICC.

Research Category: Signaling Research Sub-Category: General Post-translation Modification
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17-499
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Description
Catalogue Number17-499
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPhosphorylation Explorer Pack Sample Kit
Background Information4G10® Platinum
The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.

4A4
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
References
Product Information
Components
  • This product contains the following components:
  • Cat. # 05-1050X 4G10® Platinum, Anti-phosphotyrosine
  • Cat. # 05-1000X Anti-Phosphoserine, Clone 4A4
  • Click above links for formulation, storage/handling conditions and product specific images and certificates of analysis.
Quality LevelMQ100
Applications
ApplicationThis Antibody pack contains Anti-phosphotyrosine Antibody, Anti-Phosphoserine Antibody, validated for use in WB, IF, Flow, IHC, ELISA, ICC.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Flow Cytometry
  • Immunohistochemistry
  • ELISA
  • Immunocytochemistry
Biological Information
Clone4G10® Platinum and 4A4
HostMouse
Modifications
  • Phosphorylation
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsSee individual components for storage/handling conditions.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-499 04053252009914

Documentation

Related Products & Applications

Product Families

Phosphotyrosine Signaling Solutions

Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
Learn More >>

Phosphoserine/Threonine Antibodies & Kits

Approximately 400 of the 518 kinases in the human genome are serine/threonine specific protein kinases. Millipore offers antibodies for your phospho-Ser/Thr research needs, such as those below.
Learn More >>

Phosphoserine Antibodies

Millipore’s phosphoserine antibodies, including the popular 4A4 clone, demonstrate sensitivity and detect multiple serine phosphorylations on numerous substrates. See below for pSer antibodies.
Learn More >>

Phosphotyrosine Antibodies

Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
Learn More >>

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Life Science Research > Antibodies and Assays > Antibody Kits