Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

CC325-2 PhotoGel®-RUT, Methacrylated Gelatin Hydrogel Kit

Photocrosslinked gelatin hydrogel used for 3D cell culture and as a bioink for bioprinting (CC325 Kit Component 2)

View Products on Sigmaaldrich.com
CC325-2
1 Kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberCC325-2
DescriptionPhotoGel®-RUT, Methacrylated Gelatin Hydrogel Kit
Overview3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks.

The PhotoGel®-RUT, Methacrylated Gelatin Hydrogel Kit (CC325: CC325-1, CC325-2) is based upon purified porcine gelatin methacrylate (GelMA), which when photocrosslinked provides a native-like 3D environment for cells. Gelatin derived from denatured collagen retains many natural cell binding motifs such as RGD and MMP sites. In addition to porcine gelatin methacrylate, the kit includes the photoinitiator ruthenium/sodium persulfate for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). Gelatin methacrylate is produced from porcine, type A, 300 bloom gelatin. Gelatin macromers containing primary amino groups were reacted with methacrylic anhydride (MA) to add methacrylate pendant groups. The gelatin methacrylate achieves a degree of substitution of >70% for maximum crosslinking and range of stiffness.

References

1) Mikos, A, et al. A high-throughput approach to compare the biocompatibility of candidate bioink formulations. Bioprinting Volume 17, March 2020.

PhotoGel® is a registered trademark of Advanced BioMatrix, Inc.
Alternate Names
  • Gelatin Methacrylate
  • Gelatin Methacryloyl
  • GelMA
  • Gelatin Hydrogel
  • Gelatin Bioink
References
Product Information
Components
  • CC325-2 (Store at -20°C): Methacrylated Gelatin, 2 X 500 mg (CS226451).
  • Note: The complete PhotoGel®-RUT, Methacrylated Gelatin Hydrogel Kit (CC325) consists of CC325-1 and CC325-2 sold seperately.
Applications
ApplicationPhotocrosslinked gelatin hydrogel used for 3D cell culture and as a bioink for bioprinting (CC325 Kit Component 2)
Key Applications
  • Cell Culture
Application NotesPhotocrosslinking is initiated using a 400-450 nm light source.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceAppearance: Lyophilized powder
Gelatin Content: ≥ 1 gram
Sterility (USP modified): No Growth
pH: 6.0-8.0
Grafting Efficiency: ≥ 75%
Cell Compatibility: > 70% Cell Viability
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
CC325-2 04061842348145