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CC130 PluriSTEM-XF™ Recombinant Vitronectin

Human pluripotent stem cell culture.

PluriSTEM-XF™ Recombinant Vitronectin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Attachment, Cell Culture, Stem Cell Culture
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CC130
500 µg  
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Overview

Replacement Information

Key Specifications Table

Key Applications
Cell Attachment, CULT, Cult
Description
Catalogue NumberCC130
Trade Name
  • PluriSTEM
DescriptionPluriSTEM-XF™ Recombinant Vitronectin
Alternate Names
  • S-protein
  • Serum-spreading factor
  • V75
Background InformationPluriSTEM™ Recombinant Vitronectin is an effective xeno-free alternative to matrigel™ and has been validated to successully culture human ES and iPS cells using PluriSTEM™ Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM™ Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
References
Product Information
HS Code3821 00 00
PresentationProduct is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol.
Quality LevelMQ100
Applications
ApplicationHuman pluripotent stem cell culture.
Key Applications
  • Cell Attachment
  • Cell Culture
  • Stem Cell Culture
Application NotesSupports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
Biological Information
Concentration0.5 mg/mL
HostE. coli
PurityProduct is ≥ 92% pure by SDS-PAGE.
Gene Symbol
  • VTN
UniProt Number
Stem Cell Type
  • Human Embryonic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEach lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
CC130 04053252938511

Documentation

PluriSTEM-XF™ Recombinant Vitronectin SDS

Title

Safety Data Sheet (SDS) 

PluriSTEM-XF™ Recombinant Vitronectin Certificates of Analysis

TitleLot Number
PluriSTEM-XF™ Recombinant Vitronectin -CC130 CC130
PluriSTEM-XF™ Recombinant Vitronectin Extracellular Matrix Protein VP1709063
PluriSTEM-XF™ Recombinant Vitronectin Extracellular Matrix Protein VP1801183
PluriSTEM-XF™ Recombinant Vitronectin Extracellular Matrix Protein VP1806042

Brochure

Title
Simplifying the iPSC Workflow

Technical Info

Title
Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

Data Sheet

Title
Data Sheet - CC130