Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

529625 Polyphosphoinositide-Binding Peptide, PBP10 - CAS 794466-45-8 - Calbiochem

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: RhoB-GS160-169, Rhodamine B-QRLFQVKGRR-OH

View Products on Sigmaaldrich.com
529625
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
794466-45-8C₈₄H₁₂₇N₂₄O₁₅

Products

Catalog NumberPackaging Qty/Pack
529625-1MG Glass bottle 1 mg
Description
OverviewA Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs.
Catalogue Number529625
Brand Family Calbiochem®
SynonymsRhoB-GS160-169, Rhodamine B-QRLFQVKGRR-OH
References
ReferencesBucki, R., et al. 2001. Biochemistry 40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem. 276, 43390.
Product Information
CAS number794466-45-8
ATP CompetitiveN
FormPinkish red lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₈₄H₁₂₇N₂₄O₁₅
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetSpecifically binds to polyphosphoinositide (PPI)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Peptide SequenceRho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
529625-1MG 04055977269284

Documentation

Polyphosphoinositide-Binding Peptide, PBP10 - CAS 794466-45-8 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Polyphosphoinositide-Binding Peptide, PBP10 - CAS 794466-45-8 - Calbiochem Certificates of Analysis

TitleLot Number
529625

References

Reference overview
Bucki, R., et al. 2001. Biochemistry 40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem. 276, 43390.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-August-2008 RFH
SynonymsRhoB-GS160-169, Rhodamine B-QRLFQVKGRR-OH
DescriptionA Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.
FormPinkish red lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
CAS number794466-45-8
Peptide SequenceRho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH
Purity≥95% by HPLC
SolubilityDMSO (2 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesBucki, R., et al. 2001. Biochemistry 40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem. 276, 43390.