Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

71092 PopCulture® Reagent

PopCulture® Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
71092
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71092-3 Plastic ampoule 15 ml
71092-4 Plastic ampoule 75 ml
71092-5 Plastic ampoule 250 ml
Description
OverviewPopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium. Using this method, cell culture, protein extraction and purification performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix:target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix. Purification of fusion proteins from total culture extracts has been demonstrated using both IMAC and GST affinity chromatography methods (1).

To further enhance the PopCulture purification procedure, lysozyme and/or Benzonase® Nuclease may be added. Lysozyme cleaves a peptidoglycan bond in the E. coli cell wall, enhancing cell lysis and increasing the yield of protein (1, 2). Proteins may be expressed in a host encoding T7 lysozyme (pLysS host) or exogenous lysozyme may be added after the PopCulture Reagent. Benzonase Nuclease may also be added to degrade endogenous nucleic acids that may interfere with purification due to high viscosity.

The PopCulture protein purification procedure is ideally suited to high throughput (HT) robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience. The magnetic agarose capture resins (e.g. GST•Bind™ Magnetic Agarose Beads, His•Bind® Magnetic Agarose Beads) are optimal for HT applications since the entire procedure can be performed in a single tube without the need for columns or centrifugation.

*patent pending
Catalogue Number71092
Brand Family Novagen®
References
References1. Grabski, A., Drott, D., Handley, M., Mehler, M. and Novy, R. (2001). inNovations 13, 1–4.

2. Inouye, M., Arnheim, N. and Sternglanz, T. (1973). J. Biol. Chem. 248, 7247.
Product Information
DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Irritant
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71092-3 04055977271799
71092-4 04055977271812
71092-5 04055977271829

Documentation

PopCulture® Reagent SDS

Title

Safety Data Sheet (SDS) 

PopCulture® Reagent Certificates of Analysis

TitleLot Number
71092

References

Reference overview
1. Grabski, A., Drott, D., Handley, M., Mehler, M. and Novy, R. (2001). inNovations 13, 1–4.

2. Inouye, M., Arnheim, N. and Sternglanz, T. (1973). J. Biol. Chem. 248, 7247.

Brochure

Title
Overnight Express Autoinduction Systems
Protein Purification and Detection Tools

Citations

Title
  • Andrew T. Anfora and Rodney A. Welch. (2006) DsdX is the second D-serine transporter in uropathogenic Escherichia coli clinical isolate CFT073. Journal of Bacteriology 188, 6622-6628.
  • Ilya G. Serebriiskii, et al. (2005) A combined yeast/bacterial two-hybrid system: development and evaluation. Molecular and Cellular Proteomics 4, 819-826.
  • Henry Nguyen, et al. (2004) An automated small-scale protein expression and purification screening provides beneficial information for protein production. Journal of Structural and Functional Genomics 5, 23-27.
    		•

    User Protocols

    Title
    TB323 PopCulture® Reagent