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539253 Protein A, Peroxidase Conjugate

This Protein A, Peroxidase Conjugate is validated for use in Direct Immunoassay.

Protein A, Peroxidase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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539253
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
539253-1MG Plastic ampoule 1 mg
Description
OverviewNative protein A conjugated to horseradish peroxidase.
Catalogue Number539253
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
Preservative0.02% thimerosal
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesDirect Immunoassay (1:10,000)
Application Commentso-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
PuritySingle band 50,000 on SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
539253-1MG 07790788051310

Documentation

Protein A, Peroxidase Conjugate SDS

Title

Safety Data Sheet (SDS) 

Protein A, Peroxidase Conjugate Certificates of Analysis

TitleLot Number
539253
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2007 RFH
ApplicationDirect Immunoassay (1:10,000)
DescriptionNative protein A from Staphylococcus aureus conjugated to horseradish peroxidase.
BackgroundProtein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors and therefore will not give high background reactions.
FormLiquid
FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
Concentration Label Please refer to vial label for lot-specific concentration
PuritySingle band 50,000 on SDS-PAGE
Preservative0.02% thimerosal
Commentso-Phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C was used as a substrate for testing conjugate enzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling