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539322 Protein G, Peroxidase Conjugate

This Protein G, Peroxidase Conjugate is validated for use in Direct Immunoassay.

Protein G, Peroxidase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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539322
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
539322-500UG Plastic ampoule 500 μg
Description
OverviewPurified recombinant protein G conjugated to peroxidase. Binds ≥ 99% to human IgG immobilized on a column.
Catalogue Number539322
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 0.01 M PBS, pH 7.6, 10% mannose, 2% sucrose, 0.5 mM EDTA, 1% BSA, 0.1% Kathon
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesDirect Immunoassay (1:5000)
Application CommentsBinds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
PuritySingle band at 33.8 kDa by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
539322-500UG 04055977269086

Documentation

Protein G, Peroxidase Conjugate SDS

Title

Safety Data Sheet (SDS) 

Protein G, Peroxidase Conjugate Certificates of Analysis

TitleLot Number
539322
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-March-2021 JSW
ApplicationDirect Immunoassay (1:5000)
DescriptionPurified recombinant protein G conjugated to peroxidase.
BackgroundProtein G is a cell-surface protein of Streptococcus species that binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. Protein G is especially valued because of its ability to react with Human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e. bovine, goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE and IgM.
FormLiquid
FormulationIn 0.01 M PBS, pH 7.6, 10% mannose, 2% sucrose, 0.5 mM EDTA, 1% BSA, 0.1% Kathon
Concentration Label Please refer to vial label for lot-specific concentration
PuritySingle band at 33.8 kDa by SDS-PAGE
CommentsBinds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling