Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

IP05 Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Protein G Plus/Protein A Agarose Suspension MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
IP05
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
IP05-1.5ML Plastic ampoule 1.5 ml
Description
OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Catalogue NumberIP05
Brand Family Calbiochem®
References
Product Information
FormLiquid slurry
Formulation33% slurry in PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
IP05-1.5ML 04055977220223

Documentation

Protein G Plus/Protein A Agarose Suspension SDS

Title

Safety Data Sheet (SDS) 

Protein G Plus/Protein A Agarose Suspension Certificates of Analysis

TitleLot Number
IP05

Citations

Title
  • Ellen J. Tisdale and Cristina R. Artalejo. (2006) Src-dependent a protein kinase C(aPKC) λ tyrosine phosphorylation is required for aPKCl/λ association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. Journal of Biological Chemistry 281, 8436-8442.
  • Catherine S. Chew, et al. (2005) Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology Gastrointestinal and Liver Physiology 289, G320-G331.
  • Ellen J. Tisdale, Carmen Kelly and Cristina R. Artalejo. (2004) Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to golgi transport exclusive of its glycolytic activity. Journal of Biological Chemistry 279, 54046-54052.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision13-August-2008 JSW
    ApplicationImmunoprecipitation (see comments)
    DescriptionProtein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
    BackgroundProtein G PLUS/Protein A-Agarose mixture provides superior performance for binding of most species and subclasses of immunoglobulins. For use with rat IgM antibodies, use with goat second-step antibodies.
    FormLiquid slurry
    Formulation33% slurry in PBS.
    Preservative≤0.1% sodium azide
    CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    Citation
  • Ellen J. Tisdale and Cristina R. Artalejo. (2006) Src-dependent a protein kinase C(aPKC) λ tyrosine phosphorylation is required for aPKCl/λ association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates. Journal of Biological Chemistry 281, 8436-8442.
  • Catherine S. Chew, et al. (2005) Drebrin E2 is differentially expressed and phosphorylated in parietal cells in the gastric mucosa. American Journal of Physiology Gastrointestinal and Liver Physiology 289, G320-G331.
  • Ellen J. Tisdale, Carmen Kelly and Cristina R. Artalejo. (2004) Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to golgi transport exclusive of its glycolytic activity. Journal of Biological Chemistry 279, 54046-54052.
    		•

    Related Products & Applications

    Related Products

    Catalog Number Description
    IP02 Protein A Agarose Suspension
    IP04 Protein G Plus-Agarose Suspension
    IP08 Protein G Plus-Agarose
    IP10 Protein G Plus/Protein A-Agarose

    Categories

    Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification
    Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
    Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification