72635 Sigma-AldrichPureGenome™ Tissue DNA Extraction Kit

This product has been discontinued.

Product Description

The PureGenome™ Tissue DNA Extraction Kit is a simple procedure for the rapid isolation of total DNA (e.g. genomic, mitochondrial, parasitic, microbial, viral) from a variety of soft and solid tissues, cells and a range of biological liquids. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K, then add the specially formulated Genomic Lysis Buffer, vortex, and transfer the mixture(s) to a supplied column. The product features Fast-Spin column technology to yield high-quality, purified DNA (see Figure 1 and Figure 2 below). Enzyme inhibitors are effectively removed during the purification process. DNA purified using the PureGenome™ Tissue DNA Extraction Kit is ideally suited for Next Generation Sequencing. In addition, DNA purified using this kit can be used for Sanger-chemistry sequencing, PCR, Southern blotting, endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

This kit is capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25–35 kb.

One kit is sufficient for 50 test samples.

Panel Components

• 5 mg       PureGenome Proteinase K
• 0.5 ml     PureGenome Proteinase K Storage Buffer
• 5 ml        PureGenome 2X Digestion Buffer
• 50 ml      PureGenome Genomic Lysis Buffer
• 15 ml      PureGenome DNA-Pre-Wash Buffer
• 50 ml       PureGenome g-DNA Wash Buffer
• 10 ml       PureGenome DNA Elution Buffer
• 1 EA         PureGenome SpinIIC Columns (50 tubes/EA)
• 1 EA        PureGenome Collection Tubes (100 tubes/EA)

Storage: Store all components at room temperature prior to initial use. This kit is stable for at least one year at the recommended storage temperature. Reagents are routinely tested on lot-to-lot basis to ensure they provide maximal performance and reliability.