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540221 Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PNP

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540221
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
540221-1MG Plastic ampoule 1 mg
Description
OverviewRecombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.
Catalogue Number540221
Brand Family Calbiochem®
SynonymsPNP
References
ReferencesFilgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P<sub>i</sub> into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
FormLiquid
FormulationIn 50 mM Tris-HCl, pH 7.6.
Quality LevelMQ100
Applications
Biological Information
PuritySingle band by SDS-PAGE
Specific Activity≥25 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
540221-1MG 04055977268751

Documentation

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
540221

References

Reference overview
Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2024 JSW
SynonymsPNP
DescriptionRecombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.
FormLiquid
FormulationIn 50 mM Tris-HCl, pH 7.6.
PuritySingle band by SDS-PAGE
Specific activity≥25 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
Storage -20°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesFilgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.