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553215 RAPIDstain™ Reagent - Calbiochem

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
553215-1L Glass bottle 1 l
Description
OverviewAn ultrasensitive Coomassie-based reagent for staining polyacrylamide gels. No mixing or preparation is involved, no fixation step is needed, and no destaining is required. RAPIDstain™ Reagent stains only protein, within 5-10 min, and leaves a crystal-clear background with high resolution. Note: 1 liter is sufficient to stain up to 20 minigels or 10 standard (16 x 18 cm) gels.
Catalogue Number553215
Brand Family Calbiochem®
References
Product Information
FormLiquid
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Sensitivity4 - 8 ng BSA
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/22-36/37/38

Harmful by inhalation and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 36-45

Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
553215-1L 04055977193572

Documentation

RAPIDstain™ Reagent - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

RAPIDstain™ Reagent - Calbiochem Certificates of Analysis

TitleLot Number
553215

Technical Info

Title
Western Blotting Quick Reference Product Guide
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
DescriptionAn ultrasensitive Coomassie-based reagent for staining polyacrylamide gels. No mixing or preparation is involved, no fixation step is needed, and no destaining is required. RAPIDstain™ Reagent stains only protein, within 5-10 min, and leaves a crystal-clear background with high resolution. Contain the following component:

• RAPIDstain™ 1 x 1 liter Suitable for 20 mini gels (8 x 10 cm) or 10 larger gels (12 x 15 cm)
FormLiquid
Recommended reaction conditions1. Wash gel three times in ultra-pure water, 5-10 min per wash. NOTE: • Use 100 ml for each 8 x 10 cm mini-gel. • Isoelectric focusing gels require pre-fixing in 20% TCA for 30 min, followed by extensive washing. 2. Discard all free water from the gel. Add RAPIDStain™ to cover the gel. Gently shake gel in stain for about an h. NOTE: • Protein bands will be visible within 10 min and reach maximum intensity within 1 h. Longer incubation periods will not increase the background. 3. Wash gel two to three times in deionized water. Washing enhances the staining of weak protein bands. Store gel in water. NOTE: • If background staining is noticed then it is indicative of residual SDS in gel. Rinsing the gel extensively in ultra-pure water will remove the background staining.
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Toxicity Harmful