Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

17-439 RalB Activation Assay Kit (non-radioactive)

Non-radioactive Ral-B Activation Assay Kit can be used to precipitate Ral-B-GTP from cell lysates & detection by a Ral-B specific monoclonal antibody.

RalB Activation Assay Kit (non-radioactive) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: G-Protein Assay
View Products on Sigmaaldrich.com
17-439
20 assays  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey Applications
H, M, RGPA
Description
Catalogue Number17-439
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRalB Activation Assay Kit (non-radioactive)
Alternate Names
  • RAS-like protein B
  • v-ral simian leukemia viral oncogene homolog B
  • GTP binding protein
Materials Required but Not DeliveredStimulated and Non-stimulated Cell Lysates
Pipettes, Micropipettes and Tips
Electrophoresis and Immunoblotting Equipment
Microcentrifuge
Microcentrifuge Tubes
Ral Activators
Tissue Culture Supplies
4C Tube Rocker
Glycerol
aprotinin
PMSF
Anti-Mouse Secondary Antibody and Detection Reagents
TRIS Buffered Saline (TBS), Ice Cold
References
Product Information
Components
  • anti-RalB, clone 25 (Cat.# 04-037)
  • Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
Quality LevelMQ100
Applications
ApplicationNon-radioactive Ral-B Activation Assay Kit can be used to precipitate Ral-B-GTP from cell lysates & detection by a Ral-B specific monoclonal antibody.
Key Applications
  • G-Protein Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
Entrez Gene Number
Gene Symbol
  • RALBP1
  • RLIP1
  • RIP1
  • RalBP1
  • RIP
  • RLIP76
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15311 # Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
SIZE: 655 amino acids; 76063 Da
SUBUNIT: Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDC2 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
SIMILARITY: SwissProt: Q15311 ## Contains 1 Rho-GAP domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by non-radioactive Kinase Assay.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C from date of shipment
Packaging Information
Material Size20 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-439 04053252730849

Documentation

RalB Activation Assay Kit (non-radioactive) SDS

Title

Safety Data Sheet (SDS) 

RalB Activation Assay Kit (non-radioactive) Certificates of Analysis

TitleLot Number
RalB Activation Assay Kit (non-radioactive) - 2390479 2390479
RalB Activation Assay Kit (non-radioactive) - 2433420 2433420
RalB Activation Assay Kit (non-radioactive) - 2330561 2330561
RalB Activation Assay Kit (non-radioactive) - 2925322 2925322
RalB Activation Assay Kit (non-radioactive) - DAM1821249 DAM1821249
RalB Activation Assay Kit (non-radioactive) - R0610D0003 R0610D0003
RalB Activation Assay Kit - 3181150 3181150
RalB Activation Assay Kit - 3543348 3543348
RalB Activation Assay Kit - 4005396 4005396
RalB Activation Assay Kit - DAM1514141 DAM1514141
Show more

Brochure

Title
Hallmarks of Aging