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553210 Rapamycin - CAS 53123-88-9 - Calbiochem

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Rapamycin - CAS 53123-88-9 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: mTOR Inhibitor I

Primary Target: Mammalian target of rapamycin (mTOR)
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
53123-88-9C₅₁H₇₉NO₁₃

Products

Catalog NumberPackaging Qty/Pack
553210-100UG Plastic ampoule 100 μg
553210-10MG Glass bottle 10 mg
553210-1MG Plastic ampoule 1 mg
553210-5MG Glass bottle 5 mg
Description
OverviewAnti-fungal and immunosuppressant. Selectively inhibits the mammalian target of rapamycin (mTOR) and blocks the subsequent activation of p70 S6 kinase (IC50 = 50 pM). Specifically inhibits mTORC1, but mTORC2 that phosphorylates Akt at Ser473 appears to be insensitive to rapamycin. Prevents the translational activation of IGF-II. Also prevents resting T-cells from entering the cell cycle, but does not directly arrest cell cycle progression. Shown to inhibit later signaling events, such as p110Rb phosphorylation, p34cdc2 kinase activation, and cyclin A synthesis. Exhibits strong binding to FK-506 binding proteins. Also reported to induce apoptosis in a murine B-cell line, to inhibit lymphokine-induced cell proliferation at the G1 phase, and to irreversibly arrest Saccharomyces cerevisiae cells in the G1 phase. A 5 mM (500 µg/109 µl) solution of Rapamycin (Cat. No. 553211) in DMSO and a 10 mM (1 mg/109 µl) solution of Rapamycin (Cat. No. 553212) in EtOH is also available.
Catalogue Number553210
Brand Family Calbiochem®
SynonymsmTOR Inhibitor I
References
ReferencesChen, T., et al. 2011. Aging Cell. 10, 908.
Powell, D.J., et al. 2003. Mol. cell Biol. 23, 7794.
Braun, W., et al. 1995. FASEB J. 9, 63.
Nielsen, F.C., et al. 1995. Nature 377, 358.
Aagaard-Tillery, K.M. and Jelinek, D.F. 1994. Cell. Immunol. 156, 493.
Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
Morice, W.G., et al. 1993. J. Biol. Chem. 268, 3734.
Terada, N., et al. 1993. J. Biol. Chem. 268, 12062.
Kuo, J., et al. 1992. Nature 358, 70.
Price, D.J., et al. 1992. Science 257, 973.
Heitman, J., et al. 1991. Science 253, 905.
Kay, J.E., et al. 1991. Immunology 72, 544.
Schreiber, S.L. 1991. Science 251, 283.
Bierer, B.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 9231.
Dumont, F.J., et al. 1990. J. Immunol. 144, 251.
Product Information
CAS number53123-88-9
ATP CompetitiveN
FormClear film
Hill FormulaC₅₁H₇₉NO₁₃
Chemical formulaC₅₁H₇₉NO₁₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
Biological Information
Primary TargetMammalian target of rapamycin (mTOR)
Primary Target IC<sub>50</sub>50 pM against p70 S6 kinase
Purity≥99% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSVE6250000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
553210-100UG 04055977193510
553210-10MG 07790788060305
553210-1MG 04055977193480
553210-5MG 04055977193534

Documentation

Rapamycin - CAS 53123-88-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Rapamycin - CAS 53123-88-9 - Calbiochem Certificates of Analysis

TitleLot Number
553210

References

Reference overview
Chen, T., et al. 2011. Aging Cell. 10, 908.
Powell, D.J., et al. 2003. Mol. cell Biol. 23, 7794.
Braun, W., et al. 1995. FASEB J. 9, 63.
Nielsen, F.C., et al. 1995. Nature 377, 358.
Aagaard-Tillery, K.M. and Jelinek, D.F. 1994. Cell. Immunol. 156, 493.
Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
Morice, W.G., et al. 1993. J. Biol. Chem. 268, 3734.
Terada, N., et al. 1993. J. Biol. Chem. 268, 12062.
Kuo, J., et al. 1992. Nature 358, 70.
Price, D.J., et al. 1992. Science 257, 973.
Heitman, J., et al. 1991. Science 253, 905.
Kay, J.E., et al. 1991. Immunology 72, 544.
Schreiber, S.L. 1991. Science 251, 283.
Bierer, B.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 9231.
Dumont, F.J., et al. 1990. J. Immunol. 144, 251.

Citations

Title
  • Chen, T., et al. 2011. Aging Cell. 10, 908.
  • Yu, YL., et al. 2005. Journal of Biological Chemistry 280, 29533.
  • Vega, R.B., et al. 2004. Molecular and Cellular Biology 24, 8374.
  • Liao, X., et al. 2003. Endocrinology 144, 1656.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision11-September-2014 JSW
    SynonymsmTOR Inhibitor I
    DescriptionSelectively inhibits the mammalian target of rapamycin (mTOR) and blocks the subsequent activation of p70 S6 kinase (IC50 = 50 pM). Specifically inhibits mTORC1, but mTORC2 that phosphorylates Akt at Ser473 appears to be insensitive to rapamycin. Prevents the translational activation of IGF-II. Also prevents resting T-cells from entering the cell cycle, but does not directly arrest cell cycle progression. Shown to inhibit later signaling events, such as p110Rb phosphorylation, p34cdc2 kinase activation, and cyclin A synthesis. Exhibits strong binding to FK-506 binding proteins. Also reported to induce apoptosis in a murine B-cell line, to inhibit lymphokine-induced cell proliferation at the G1 phase, and to irreversibly arrest Saccharomyces cerevisiae cells in the G1 phase.
    FormClear film
    CAS number53123-88-9
    RTECSVE6250000
    Chemical formulaC₅₁H₇₉NO₁₃
    Structure formulaStructure formula
    Purity≥99% by HPLC
    SolubilityDMSO (200 mg/ml) or Ethanol (50 mg/ml)
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Standard Handling
    Merck USA index14, 8114
    ReferencesChen, T., et al. 2011. Aging Cell. 10, 908.
    Powell, D.J., et al. 2003. Mol. cell Biol. 23, 7794.
    Braun, W., et al. 1995. FASEB J. 9, 63.
    Nielsen, F.C., et al. 1995. Nature 377, 358.
    Aagaard-Tillery, K.M. and Jelinek, D.F. 1994. Cell. Immunol. 156, 493.
    Gottschalk, A.R., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 7350.
    Morice, W.G., et al. 1993. J. Biol. Chem. 268, 3734.
    Terada, N., et al. 1993. J. Biol. Chem. 268, 12062.
    Kuo, J., et al. 1992. Nature 358, 70.
    Price, D.J., et al. 1992. Science 257, 973.
    Heitman, J., et al. 1991. Science 253, 905.
    Kay, J.E., et al. 1991. Immunology 72, 544.
    Schreiber, S.L. 1991. Science 251, 283.
    Bierer, B.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 9231.
    Dumont, F.J., et al. 1990. J. Immunol. 144, 251.
    Citation
  • Chen, T., et al. 2011. Aging Cell. 10, 908.
  • Yu, YL., et al. 2005. Journal of Biological Chemistry 280, 29533.
  • Vega, R.B., et al. 2004. Molecular and Cellular Biology 24, 8374.
  • Liao, X., et al. 2003. Endocrinology 144, 1656.
    		•