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17-218 Ras Activation Assay Kit

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17-218
1 kit  Kit capacity: 30 assays
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Overview

Replacement Information

Key Specifications Table

Key Applications
Enzyme Assays
Description
Catalogue Number17-218
ReplacesSGT435
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRas Activation Assay Kit
References
Product Information
Components
  • Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)
  • Anti-Ras, clone RAS10 (Cat.# 05-516)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Quality LevelMQ100
Applications
Key Applications
  • Enzyme Assays
Biological Information
Entrez Gene Number
Entrez Gene SummaryMembers of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
Gene Symbol
  • MRAS
  • M-RAs
  • M-RAS
  • FLJ42964
  • R-RAS3
  • RRAS3
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14807 # May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
SIZE: 208 amino acids; 23846 Da
SUBUNIT: Interacts with RGL3 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
TISSUE SPECIFICITY: Expression highly restricted to the brain and heart.
SIMILARITY: SwissProt: O14807 ## Belongs to the small GTPase superfamily. Ras family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThis kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Material PackageKit capacity: 30 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-218 04053252516689

Documentation

Ras Activation Assay Kit SDS

Title

Safety Data Sheet (SDS) 

Ras Activation Assay Kit Certificates of Analysis

TitleLot Number
Ras Activation Assay Kit (non-radioactive) - 2390546 2390546
Ras Activation Assay Kit (non-radioactive) 2834455
Ras Activation Assay Kit (non-radioactive) 3020728
Ras Activation Assay Kit (non-radioactive) 3125714
Ras Activation Assay Kit (non-radioactive) 2943900
Ras Activation Assay Kit (non-radioactive) 3099095
Ras Activation Assay Kit (non-radioactive) 2872357
Ras Activation Assay Kit (non-radioactive) - 1959294 1959294
Ras Activation Assay Kit (non-radioactive) - 2166054 2166054
Ras Activation Assay Kit (non-radioactive) - 2210394 2210394

References

Reference overviewApplicationPub Med ID
The TGFβ receptor-interacting protein km23-1/DYNLRB1 plays an adaptor role in TGFβ1 autoinduction via its association with Ras.
Jin, Q; Ding, W; Mulder, KM
The Journal of biological chemistry  287  26453-63  2012

Show Abstract
22637579 22637579
E4orf1: a novel ligand that improves glucose disposal in cell culture.
Dhurandhar, EJ; Dubuisson, O; Mashtalir, N; Krishnapuram, R; Hegde, V; Dhurandhar, NV
PloS one  6  e23394  2011

Show Abstract Full Text Article
21886789 21886789
Effects of estrogen on stress-induced premature senescence of vascular smooth muscle cells: A novel mechanism for the \time window theory\ of menopausal hormone therapy.
Zhu C, Zhang L, Zheng Y, Xu J, Song J, Rolfe BE, Campbell JH
Atherosclerosis  215  294-300. Epub 2011 Jan 19.  2011

21300357 21300357
Ligand-specific function of transforming growth factor beta in epithelial-mesenchymal transition in heart development.
Azhar, M; Runyan, RB; Gard, C; Sanford, LP; Miller, ML; Andringa, A; Pawlowski, S; Rajan, S; Doetschman, T
Developmental dynamics : an official publication of the American Association of Anatomists  238  431-42  2009

Show Abstract Full Text Article
19161227 19161227
Gene silencing for epidermal growth factor receptor variant III induces cell-specific cytotoxicity.
Yamoutpour, Farnaz, et al.
Mol. Cancer Ther., 7: 3586-97 (2008)  2008

Show Abstract
19001441 19001441
Activated K-RAS increases polyamine uptake in human colon cancer cells through modulation of caveolar endocytosis.
Upal K Basu Roy,Nathaniel S Rial,Karen L Kachel,Eugene W Gerner
Molecular carcinogenesis  47  2008

Show Abstract Full Text Article
18176934 18176934
RIG1 suppresses Ras activation and induces cellular apoptosis at the Golgi apparatus.
Tsai, Fu-Ming, et al.
Cell. Signal., 19: 989-99 (2007)  2007

Show Abstract
17196792 17196792
RIG1 inhibits the Ras/mitogen-activated protein kinase pathway by suppressing the activation of Ras.
Tsai, Fu-Ming, et al.
Cell. Signal., 18: 349-58 (2006)  2006

Show Abstract
16005186 16005186
Optimization of a nonradioactive method for consistent and sensitive determination of activated K-ras protein.
Richard J Calvert,Wafa Kammouni,Keith D Kikawa
Analytical biochemistry  343  2005

Show Abstract
16018961 16018961
Methylation and inhibition of expression of uPA by the RAS oncogene: divergence of growth control and invasion in breast cancer cells.
Pouya Pakneshan, Moshe Szyf, Shafaat A Rabbani
Carcinogenesis  26  557-64  2005

Show Abstract
15618232 15618232

Brochure

Title
Hallmarks of Aging

Related Products & Applications

Accessories

Catalog Number Description
05-1072 Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2

Kit Component

Catalog Number Description
05-516 Anti-Ras Antibody, clone RAS10
14-278 Ras Assay Reagent (Raf-1 RBD, agarose)
20-168 Mg2+ Lysis/Wash Buffer, 5X
20-176 100X GTPγS, 10mM
20-177 100X GDP, 100mM

Product Families

Categories

Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays