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HTS223RTA Ready-to-Assay™ D4 Dopamine Receptor Frozen Cells

Human D4 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-5 Entrez Gene Number: NM_000797
HTS223RTA
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

SpeciesHost CellsProtein Target
Human Chem-5D4
Description
Catalogue NumberHTS223RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ D4 Dopamine Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The neurotransmitter dopamine is involved in a wide variety of neuroendocrine, emotional, cognitive and locomotor functions. These activities of dopamine are mediated by a group of 5 G protein coupled receptors, 2 of which (D1 and D5) couple to Gs and 3 of which (D2, D3, D4) couple to Gi (Missale et al., 1998). The D4 receptor is highly polymorphic, particularly in the third cytoplasmic loop, which contains from 2 to 7 repeats of 16 amino acids. Individuals with 7 repeats have increased risk of developing ADHD (Thapar et al., 2005). Mice engineered to lack D4 have reduced locomotor activity, increased sensitivity to drugs of abuse that elevate dopamine (cocaine, methamphetamine and ethanol), and reduced response to novelty (Rubinstein et al., 1997; Dulawa et al., 1999). Millipore’s cloned human D4-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant D4 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at D4.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman D4 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-5
Protein TargetD4
Target Sub-FamilyDopamine
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HTS223RTA 04053252552434

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Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells