Ready-to-Assay FFA2 / GPR43 Free Fatty Acid Family Receptor Frozen Cells

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

FFA2, also known as GPR43, is a GPCR that, along with GPR41, is activated by short chain carboxylic acids formate, acetate, proprionate, butyrate and pentanoate. Neutrophils and other leukocytes selectively express FFA2, and FFA2 mediates calcium flux and chemotaxis in these cells by coupling to both Gq and Gi (Brown et al., 2003; Le Poul et al., 2003). Millipore′s cloned human FFA2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FFA2 expression on the cell surface for robust signaling via the calcium pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at FFA2.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

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