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HTS135RTA Ready-to-Assay™ FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells

Human FFA3 / GPR41 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-4 Entrez Gene Number: NM_005304
HTS135RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Key Specifications Table

SpeciesHost CellsProtein Target
Human Chem-4FFA3 / GPR41
Description
Catalogue NumberHTS135RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

GPR41 is a GPCR that, along with GPR43, is activated by short chain carboxylic acids formate, acetate, proprionate, butyrate and pentanoate (Brown et al., 2003; Brown et al., 2005). Binding of these ligands to GPR41 selectively activates Gi to inhibit cAMP accumulation. Expression of GPR41 is prominent in adipose tissue, increases during differentiation of cultured adipocytes, and allows short chain carboxylic acids to stimulate leptin synthesis in adipocytes (Xiong et al., 2003) Millipore’s cloned human GPR41-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant GPR41 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GPR41 (FFA3).
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman FFA3 / GPR41 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-4
Protein TargetFFA3 / GPR41
Target Sub-FamilyFree Fatty Acid
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HTS135RTA 04053252481598

Documentation

Ready-to-Assay™ FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells SDS

Title

Safety Data Sheet (SDS) 

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Categories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells