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HTS173RTA Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells

Human PTH2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_005048
HTS173RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

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Key Specifications Table

SpeciesHost CellsProtein Target
Human Chem-1PTH2
Description
Catalogue NumberHTS173RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Parathyroid hormone (PTH) is recognized by three different class B GPCRs; PTH1, PTH2, and PTH3, which couple to Gs to stimulate cAMP production. The PTH1 receptor is found in high levels in the kidney and bone, where it binds PTH and PTHrP to regulate Ca2+ homeostasis (Brown et al, 1996). The PTH2 receptor, which binds PTH and the neuropeptide tuberoinfundibular peptide 39 (TIP-39) but not PTHrP, has about 50% amino acid sequence identity to the PTH1 receptor. PTH2 is found in the greatest volume in the nervous system and at a very low density in the kidney and bone (Usdin et al, 1995). The PTH3 receptor, which has been recently discovered in the zebrafish, has 61% amino acid identity to the zebrafish PTH1 receptor and also seems to share its ligand affinity (Rubin et al, 1999). Studies with the selective PTH2 receptor agonist, TIP-39, and the distribution of the receptor in the superficial dorsal horn of the spinal cord suggest the receptor may play a role in pain perception (Usdin et al, 1999). Millipore’s cloned human PTH2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at PTH2.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman PTH2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
Key Applications
  • Calcium Flux Assays
  • cAMP Assays
Biological Information
Entrez Gene Number
GPCR ClassB
Host CellsChem-1
Protein TargetPTH2
Target Sub-FamilyPTH receptor
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HTS173RTA 04053252630057

Documentation

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Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells