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HTS053RTA Ready-to-Assay™ XCR1 Chemokine Family Receptor Frozen Cells

Human XCR1 / GPR5 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-4 Entrez Gene Number: NM_005283
HTS053RTA
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

SpeciesHost CellsProtein Target
Human Chem-4XCR1 / GPR5
Description
Catalogue NumberHTS053RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ XCR1 Chemokine Family Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The migration of leukocytes from the bloodstream to sites of inflammation is a dynamic factor involving adhesion molecules and chemotactic factors. Chemokines play a role in the trafficking of leukocytes by inducing cellular motility and activating adhesion molecules within the immune system. Lymphotactin (also known as XCL1 and SCM-1) is a unique chemokine that retains only two of the four cysteine residues found in the CC, CXC and CX3C families of chemokines. A Gi-coupled receptor, XCR1, binds to lymphotactin and mediates its chemotactic effects (Yoshida et al., 1998). Chemokines promote accumulation of activated mononuclear cells (MNCs) in inflamed joints in rheumatoid arthritis (RA) and lymphotacin is highly expressed in synovial fluid of RA patients. In situ hybridization studies indicate that XCR1 expression was detected in both the infiltrated MNCs and the synoviocytes from synovial specimens taken from RA patients (Wang et al., 2004). Millipore’s cloned human XCR1-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant XCR1 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at XCR1.
References
Product Information
Components
  • Pack contains 1 vial of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman XCR1 / GPR5 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-4
Protein TargetXCR1 / GPR5
Target Sub-FamilyChemokine
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HTS053RTA 04053252552618

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