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14-480M Rsk2/MAPKAP Kinase 1b Protein, active, 250 µg

Active, recombinant human RSK2, full-length with an N-terminal His6 tag between Met1 & Pro2, for use in Kinase Assays.

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14-480M
250 µg  Also available in 10 μg size (cat#14-480) and in bulk (cat# 14-480-K).
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Overview

Replacement Information
Description
Catalogue Number14-480M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRsk2/MAPKAP Kinase 1b Protein, active, 250 µg
OverviewRecombinant human RSK2, full-length with an N-terminal His6 tag between Met1 and Pro2
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, recombinant human RSK2, full-length with an N-terminal His6 tag between Met1 & Pro2, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
Gene Symbol
  • RPS6KA3
  • CLS
  • RSK-2
  • RSK2
  • p90-RSK2
  • ISPK1
  • MRX19
  • HU-3
  • pp90RSK2
  • MAPKAPK1B
  • HU-2
  • RSK
  • S6K-alpha3
  • ISPK-1
Protein TargetRsk2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P51812 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
COFACTOR: Magnesium (By similarity).
SIZE: 740 amino acids; 83736 Da
SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity).
TISSUE SPECIFICITY: Expressed in many tissues, highest levels in skeletal muscle.
PTM: Autophosphorylated on Ser-386, as part of the activation process (By similarity). & Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369 (By similarity).
DISEASE: SwissProt: P51812 # Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
SIMILARITY: SwissProt: P51812 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains.
Molecular Weight88kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurancethe MAPKAP Kinase 2 substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-480) and in bulk (cat# 14-480-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-480M 04053252322211

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-480 Rsk2/MAPKAP Kinase 1b Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases