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03-230 SIRT5 (His-tag) human recombinant - HDAC protein

Human recombinant SIRT5 (His-tag) expressed in E. Coli.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
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03-230
500 µg  
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Overview

Replacement Information

Key Specifications Table

Key ApplicationsSpecies
Enzyme AssaysHuman
Description
Catalogue Number03-230
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSIRT5 (His-tag) human recombinant - HDAC protein
OverviewHuman recombinant SIRT5 (His-tag) expressed in E. Coli
Background InformationSIRT5, along with SIRT 3 and 4, is one of three human mitochondrial sirtuins (homologs of yeast Sir2). SIRT5, which is localized to both the mitochondrial matrix and the intermembrane space, is an NAD+-dependent lysine deacetylase (class III HDAC). SIRT5’s only known physiological target is the intermembrane space protein, cytochrome c, suggesting the possibility of regulatory roles in metabolism and/or apoptosis. SIRT5 has also been shown to deacetylate and thereby upregulate the matrix urea cycle enzyme carbamoyl phosphate synthetase 1. This implies an important role for SIRT5 in ammonia detoxification and adaptation to elevated amino acid catabolism during starvation, calorie restriction, or a high protein diet. SIRT5 is most strongly expressed in thymus, lymphoblasts, and heart muscle cells and its chromosomal location (6p23) has been implicated in chromosomal abnormalities associated with malignancies.
References
Product Information
FormatPurified
PresentationIn liquid containing 25 mM Tris, pH 7.5, 100 mM NaCl, 5 mM DTT, containing 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant SIRT5 (His-tag) expressed in E. Coli.
Key Applications
  • Enzyme Assays
Application NotesHDAC deacetylase assays
Biological Information
HostE. coli
PurityPurity >95% by SDS-PAGE
SourceRecombinant residues 37-310 of human SIRT5 isoform 1, with an N-terminal His-tag
Specific Activity>200 pmol/min/mg at 37ºC, as determined by a fluorometric activity assay.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes before storing at -80°C.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-230 04053252550911

Documentation

SIRT5 (His-tag) human recombinant - HDAC protein SDS

Title

Safety Data Sheet (SDS) 

SIRT5 (His-tag) human recombinant - HDAC protein Certificates of Analysis

TitleLot Number
SIRT5 (His-tag) human recombinant - 3260677 3260677
SIRT5 (His-tag) human recombinant - 3501252 3501252
SIRT5 (His-tag) human recombinant - 3985806 3985806
SIRT5 (His-tag) human recombinant - 4044925 4044925
SIRT5 (His-tag) human recombinant - 4066271 4066271
SIRT5 (His-tag) human recombinant - 4094210 4094210
SIRT5 (His-tag) human recombinant - 4108311 4108311
SIRT5 (His-tag) human recombinant - 4109724 4109724
SIRT5 (His-tag) human recombinant - 4109725 4109725
SIRT5 (His-tag) human recombinant - Q2022483 Q2022483
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