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03-0200-00 SMC™ Human NGFβ High Sensitivity Immunoassay Kit

The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human NGFβ High Sensitivity Immunoassay Kit unparalleled performance in the quantification of NGFβ High Sensitivity Immunoassay in human serum and plasma samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Neuroscience Research Sub-Category: Neurofilament & Neuron Metabolism, Neuronal & Glial Markers, Neuroscience
03-0200-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey Applications
NGFβ HSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0200-00
Trade Name
  • SMC®
DescriptionSMC™ Human NGFβ High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human NGFβ High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationNerve growth factor-beta (NGFβ) is a neurotrophin that is involved in neural cell survival, growth, and differentiation into neurons, while also having a role in neuron migration. It has been shown to have pro-inflammatory and pro-fibrogenic properties, functioning through the AKT and MAP Kinase pathways. In terms of nervous system function, it has been shown to be involved in perception of pain, temperature, and touch by sensory neurons. The SMC™ Human NGFβ High Sensitivity Immunoassay Kit harnesses the power of digital Single Molecule Counting technology to enable researchers to better understand the dynamics of this protein in neuroscience research by achieving accurate and reproducible measurements of NGFβ concentrations in human serum and plasma samples.
References
Product Information
Components
  • NGFβ Coated Beads
  • Standard Diluent
  • NGFβ Detection Antibody
  • Assay Buffer
  • Human NGFβ Standard
  • SMC™ Human NGFβ Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer D
Precision, %
  • Low-end CV% Range: <20%
  • Low-end CV% Average: 80-120%
Quality LevelMQ200
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human NGFβ High Sensitivity Immunoassay Kit unparalleled performance in the quantification of NGFβ High Sensitivity Immunoassay in human serum and plasma samples.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • NGFβ
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-173%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-0200-00 04065266071214