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03-0208-00 SMC® Human PD-L1 High Sensitivity Immunoassay Kit

The SMC® Human PD-L1 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Programmed cell death protein ligand 1 (PD-L1) in Human Serum and K2EDTA Plasma samples.

03-0208-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-0208-00
Trade Name
  • SMC®
DescriptionSMC® Human PD-L1 High Sensitivity Immunoassay Kit
OverviewThe SMC® Human PD-1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Background InformationThe Single Molecule Counting (SMC®) Human PD-L1 High Sensitivity Immunoassay uses a quantitative fluorescent sandwich immunoassay technique to measure PD-L1 in Human Serum and Plasma samples. A capture antibody specific for Human PD-L1 has been pre-coated onto paramagnetic microparticles (beads). The user pipettes beads, standards, and samples into uncoated microplate wells. During incubation, the PD-L1 present in the sample binds to the capture antibody on the coated beads. Unbound molecules are washed away during the subsequent wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to PD-L1 that has been captured onto the beads, thus completing the sandwich. Elution buffer is added to dissociate the protein sandwich, releasing the fluor-labeled antibodies. The eluted antibodies are transferred to a SMCxPRO® 384-well plate. The plate is loaded into the SMCxPRO® System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of PD-L1 present in the sample. The amount of PD-L1 in unknown samples is interpolated from a standard curve.
References
Product Information
Components
  • Assay Buffer;PD-L1 Antibody Coated Beads;Standard Diluent;PD-L1 Detection Antibody;PD-L1 Standard;10X Wash Buffer;Buffer D;Elution Buffer B
Precision, %
  • The assay variations of SMC® Human PD-L1 Immunoassay kits were studied using eight normal serum and seven normal plasma samples run in triplicate by 3 different operators on 3 different days.;Mean intra-assay variation was 10%.;Mean inter-assay variation was 15%. .
Quality LevelMQ200
Applications
ApplicationThe SMC® Human PD-L1 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Programmed cell death protein ligand 1 (PD-L1) in Human Serum and K2EDTA Plasma samples.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesProgrammed cell death protein ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) and B7-H1, is a type 1 membrane protein of 290 amino acids. It is found in a variety of cell types and binds programmed cell death protein (PD-1). It has been indicated as playing a role in autoimmune disease, allograft responses, pregnancy, and other disease states. When PD-L1 binds to PD-1, an inhibitory signal is transmitted that ultimately reduces the proliferation of antigen-specific T cells and reduces the rate of apoptosis in regulatory T-cells. By doing so, PD-L1 contributes to the immune evasion of cancer tissues by suppressing the adaptive immune system. Blocking this activity is thought to restore anti-tumor activity. High PD-L1 expression is correlated with poor prognosis with some cancer types. High levels of the soluble form are also associated with poor prognosis and advanced tumor progression. Multiple PD-L1 inhibiting monoclonal antibodies have been approved as cancer therapeutics. We are proud to offer the new SMC® Human PD-L1 High Sensitivity Immunoassay Kit for use with the state-of-the-art SMCxPRO® detection system. Researchers can accurately assess PD-L1 concentrations even in low abundance in human serum and plasma samples, enabling studies that examine the relevance of this key analyte from health to disease.

This new streamline kit has the following features:

• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Rigorously developed to achieve accurate and reproducible performance on the state-of-the-art SMCxPRO® ultrasensitive immunoassay system
Biological Information
Cross ReactivityCross-reactivity to the following analyte was tested with the following result:
PD-L1: Not detected
Accuracy
  • Ten (10) neat samples (5 serum and 5 K2 EDTA plasma) were spiked with four different concentrations of recombinant PD-L1. Spike Recovery Range: Serum 108 - 143%, Plasma 70 -110%; Spike Recovery Mean: Serum 119%, Plasma 95%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.78 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-0208-00 04065270504982

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