Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

03-0196-00 SMC™ Human Total α-synuclein High Sensitivity Immunoassay Kit

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Neuroscience, neurological Research Sub-Category: Nervous System, Neuronal & Glial Markers, Neuroscience, Neurodegenerative Diseases
View Products on Sigmaaldrich.com
03-0196-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey Applications
Ca, Fe, R, MSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0196-00
Trade Name
  • SMC®
DescriptionSMC™ Human Total α-synuclein High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human Total-α-Synuclein High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Alternate Names
  • Single Molecule Counting, SMC, SMC™, Human α-synuclein, Human αsynuclein, human α-synuclein, human α-synuclein, α-synuclein, αsynuclein, SNCA, ELISA, Human total-α-synuclein, Human total-α-synuclein, total-α-synuclein, total-αsynuclein, Human a-synuclein, Human asynuclein, human a-synuclein, human a-synuclein, a-synuclein, asynuclein, Human total-a-synuclein, total-a-synuclein, total-asynuclein, Human alpha-synuclein, Human alphasynuclein, human alpha-synuclein, alpha-synuclein, alphasynuclein, Human total-alpha-synuclein, total-alpha-synuclein , total-alphasynuclein, high sensitivity
Background InformationHuman α-synuclein, encoded by the gene SNCA, is a presynaptic neuronal protein that has been studied for its potential pathological link to neurodegenerative diseases, most notably Parkinson’s disease (PD), where this protein has been observed to undergo aberrant misfolding and aggregation within neurons. The SMC™ Human Total-α-synuclein High Sensitivity Immunoassay Kit enables researchers to further their understanding of this protein as a potential neurodegenerative marker, by accurately measuring this analyte in human cerebrospinal fluid (CSF), serum, and plasma samples. Additionally, this kit can be utilized in conjunction with the SMC™ Human phospho-α-synuclein High Sensitivity Immunoassay Kit to examine the relationship between total and phospho protein levels in neurodegenerative disease research.
References
Product Information
Components
  • Assay Buffer
  • Total α-synuclein Coated Beads
  • Standard Diluent
  • Total α-synuclein Detection Antibody
  • Total α-synuclein Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • The assay variations of SMC™ Human Totalα-synuclein Immunoassay kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
  • Mean intra-assay variation was 9%.
  • Mean inter-assay variation was 14%.
Applications
ApplicationUses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has the following features:

• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Able to achieve assay specifications on both SMCxPRO™ and Erenna®.
Biological Information
Species Reactivity
  • Canine
  • Feline
  • Rat
  • Mouse
Species Reactivity NoteSpecificity to the following species samples were tested with the following
results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 100%
Beagle – 100%
Cross ReactivityCross-reactivity to the following analytes were tested with the following results:

Tau – 0%
UCHL1 – 0%
β-synuclein – 0%
γ-synuclein – 0%
Accuracy
  • CSF Recovery: 5 CSF samples spiked with average recovery of 96% and a range of 90-102%
  • Serum Recovery: 4 serum samples spiked with average recovery of 96% and a range of 93-98%
  • Plasma Recovery: 4 plasma samples spiked with average recovery of 96% and a range of 93-97%
Physicochemical Information
Sensitivity
  • The LLOQof Total α-synuclein is 1.95 pg/mL.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-0196-00 04065265393423