Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

03-0123-00 SMC™ Mouse IL-17A Discovery Immunoassay Kit

View Products on Sigmaaldrich.com
03-0123-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey Applications
IL-17A MSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0123-00
Trade Name
  • SMC®
DescriptionSMC™ Mouse IL-17A Discovery Immunoassay Kit
OverviewThe SMC™ Mouse IL-17A Discovery Immunoassay Kit contains all reagents required to perform the assays. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin-17A (IL-17A) is the founding member of a group of cytokines called the IL-17 family. There are several members of the IL-17 family, from IL-17A to IL-17F (IL-17E is also called IL-25). IL-17A is involved in mediating proinflammatory responses by inducing the production of many other cytokines, chemokines, and prostaglandins from many cell types. The increased expression of chemokines attracts other cells, including neutrophils. IL-17A has many physiological functions, such as airway remodeling. As a result of these roles, the IL-17 family has been linked to many immune/autoimmune related diseases, including rheumatoid arthritis (RA), asthma, allergic responses, lupus, allograft rejection and antitumor immunity.
References
Product Information
Components
  • Assay Plate
  • 10X PBS
  • Blocking Buffer
  • Standard Diluent
  • Mouse IL-17A Detection Antibody
  • Mouse IL-17A Standard
  • SMC™ Mouse IL-17A Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Buffer C
  • Elution Buffer B
Precision, %
  • Low-end CV% Range: 0-20%
  • Low-end CV% Average: 11%
Quality LevelMQ200
Applications
ApplicationThe Single Molecule Counting (SMC™) technology gives the SMC™ Mouse IL-17A Discovery Immunoassay Kit unparalleled performance in the quantification of IL-17A in mouse serum.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • IL-17A
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
03-0123-00 04055977317671