Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

560000 SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem

Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
560000
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

CAS #
3483-12-3

Products

Catalog NumberPackaging Qty/Pack
560000-10ML Glass bottle 10 ml
560000-1SET Glass bottle 1 set
Description
OverviewConcentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
Note: 1 set = 20 x 10 ml.
Catalogue Number560000
Brand Family Calbiochem®
References
ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry 3, 480.
May, J.R. 1953 (September). Lancet p. 535.
Product Information
CAS number3483-12-3
FormLiquid
Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSEK1610000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +15°C to +30°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
560000-10ML 04055977268195
560000-1SET 07790788051556

Documentation

SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Certificates of Analysis

TitleLot Number
560000

References

Reference overview
Hirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry 3, 480.
May, J.R. 1953 (September). Lancet p. 535.

Citations

Title
  • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision28-April-2008 RFH
    DescriptionA concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
    FormLiquid
    Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
    Recommended reaction conditionsA. To prepare sputum samples: 1. In a screw-cap centrifuge tube, overlay the sputum sample with an equal volume of the diluted SPUTOLYSIN® Reagent. 2. Suspend the sputum by mixing on a vortex mixer for 30 s. 3. Allow the suspended mixture to stand at room temperature for 15 min. 4. Centrifuge the mixture for 5 min at 1500 rpm. Discard the supernatant. B. To prepare cultures for acid-fast bacilli: 1. Suspend the pellet from Step A(4) in 5 to 10 ml of 1% sodium hydroxide by mixing on a vortex mixer for 1 min. 2. Centrifuge the mixture for 15 min at 3000 rpm. Discard the supernatant. 3. Rinse the pellet twice in 10 ml of sterile normal saline to remove the residual sodium hydroxide. 4. Resuspend the pellet in 0.5 ml of the diluted SPUTOLYSIN® Reagent.
    CAS number3483-12-3
    RTECSEK1610000
    SolubilityDilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
    Storage Protect from light
    +15°C to +30°C
    Do Not Freeze Ok to freeze
    Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
    Toxicity Harmful
    ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
    Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
    Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
    Cleland, W.W. 1964. Biochemistry 3, 480.
    May, J.R. 1953 (September). Lancet p. 535.
    Citation
  • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
    		•