Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

69232 S•Tag™ Thrombin Purification Kit

S•Tag™ Thrombin Purification Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69232
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
69232-3 Plastic ampoule 1 kit
Description
OverviewThe S•Tag™ Thrombin Purification Kit is designed for rapid affinity purification of S•Tag fusion proteins that also contain a sequence for thrombin cleavage. The S-protein Agarose specifically retains S•Tag fusion proteins. When fusion proteins are expressed from vectors that also encode a thrombin cleavage site (LVPR/GS) between the standard 15-amino acid S•Tag sequence (KETAAAKFERQHMDS) and the cloning region, thrombin digestion can release the target protein from the S-protein Agarose. This kit uses biotinylated thrombin to enable simple and specific thrombin removal with Streptavidin-Agarose, after the digestion step.
Catalogue Number69232
Brand Family Novagen®
References
Product Information
•2 mlS-protein Agarose
•3 × 5 ml10X Bind/Wash Buffer
•3 × 1 ml10X Thrombin Cleavage Buffer
•50 UBiotinylated Thrombin
•0.4 ml Streptavidin Agarose
•2 ea/pkgSpin Filters, 5 ml capacity
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
69232-3 04055977274615

Documentation

S•Tag™ Thrombin Purification Kit Certificates of Analysis

TitleLot Number
69232

Brochure

Title
Protein Purification and Detection Tools

Citations

Title
  • Christoph Edner, et al. (2007) Glucan, water dikinase activity stimulates breakdown of starch granules by plastidial beta-amylases. Plant Physiology 145, 17-28.
  • Manu Agarwal, et al. (2006) A R2R3-type myb transcription factor is involved in the cold-regulation of CBF genes and in acquired freezing tolerance. Journal of Biological Chemistry 281, 37636-37645.
  • Kunhong Xiao, et al. (2004) Activation-dependent conformational changes in β-arrestin 2. Journal of Biological Chemistry 279, 55744-55753.
    		•

    User Protocols

    Title
    TB087