Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

AC111B Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate

View Products on Sigmaaldrich.com
AC111B
200 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIHCShBiotinPolyclonal Antibody
Description
Catalogue NumberAC111B
DescriptionSheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate
Background InformationImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
References
Product Information
FormatBiotin
PresentationPurified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationSheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate is an antibody against Sheep Mouse IgG for use in Immunohistochemistry.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A representative lot was validated by immunohistochemistry staining (1:500 dilution) of human liver tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).
Biological Information
ImmunogenPurified mouse IgG.
ConcentrationPlease refer to lot specific datasheet.
HostSheep
SpecificityBased on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG2>IgG2a>IgG3>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
IsotypeIgG1
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Purification MethodFractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to biotin conjugation.
UniProt Number
Molecular Weight~150 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human prostate tissue.

Immunohistochemistry Analysis: Performance of this biotin-conjugated secondary antibody has been validated by immunohistochemistry staining (1:500 dilution) of human prostate tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AC111B 04055977344103