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566660 Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem

A cell-permeable Smoothened Agonist, SAG, CAS 364590-63-6, modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3

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566660
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
364590-63-6C₂₈H₂₈ClN₃OS • 2HCl • 2H₂O

Products

Catalog NumberPackaging Qty/Pack
566660-1MG Glass bottle 1 mg
566660-5MG Glass bottle 5 mg
Description
OverviewA cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC50 = ∼ 3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566661) in H2O is also available.
Catalogue Number566660
Brand Family Calbiochem®
SynonymsN-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3
References
ReferencesWang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
Chen, W., et al. 2004. Science 306, 2257.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.
Product Information
CAS number364590-63-6
FormLight yellow solid
Hill FormulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
Chemical formulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
566660-1MG 07790788051570
566660-5MG 04055977191578

Documentation

Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem Certificates of Analysis

TitleLot Number
566660

References

Reference overview
Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
Chen, W., et al. 2004. Science 306, 2257.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.

Citations

Title
  • Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-February-2012 RFH
    SynonymsN-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3
    DescriptionA cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC50 ∼3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations.
    FormLight yellow solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number364590-63-6
    Chemical formulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
    Structure formulaStructure formula
    Purity≥98% by HPLC
    SolubilityDMSO (10 mg/ml) or H₂O (25 mg/ml)
    Storage Protect from light
    +2°C to +8°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
    Toxicity Standard Handling
    ReferencesWang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
    Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
    Chen, W., et al. 2004. Science 306, 2257.
    Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
    Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.
    Citation
  • Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
    		•