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567704 Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: SCDase

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567704
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
567704-250MIU Plastic ampoule 250 miu
Description
OverviewNative sphingolipid ceramide N-deacylase from Pseudomonas species. Hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in various sphingolipids and gangliosides. This enzyme can also catalyze the reverse reaction and transacylation. It is not recommended for hydrolysis of ceramide. Note: 1MU = 1 milliunit.
Note: 1 mU = 1 milliunit.
Catalogue Number567704
Brand Family Calbiochem®
SynonymsSCDase
References
ReferencesMitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.
Product Information
Activity≥1 unit/ml.
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.
EC number3.5.1.-
FormLiquid
FormulationIn 50 mM sodium acetate buffer, 0.1% LUBROL® PX Detergent, pH 6.0.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Contaminantsα- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
567704-250MIU 04055977190793

Documentation

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp. SDS

Title

Safety Data Sheet (SDS) 

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp. Certificates of Analysis

TitleLot Number
567704

References

Reference overview
Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

Brochure

Title
Gangliosides Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-September-2007 RFH
SynonymsSCDase
DescriptionNative sphingolipid ceramide N-deacylase from Pseudomonas species. Hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in various sphingolipids and gangliosides. This enzyme can also catalyze the reverse reaction and transacylation. It is not recommended for hydrolysis of ceramide. Note: 1MU = 1 milliunit.
FormLiquid
FormulationIn 50 mM sodium acetate buffer, 0.1% LUBROL® PX Detergent, pH 6.0.
EC number3.5.1.-
Contaminantsα- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases: none detected
Activity≥1 unit/ml.
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.