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569397 Staurosporine, Streptomyces sp.

Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).

Staurosporine, Streptomyces sp. MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MLCK Inhibitor I, PKA Inhibitor II

Primary Target: PKA
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569397
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
62996-74-1C₂₈H₂₆N₄O₃

Products

Catalog NumberPackaging Qty/Pack
569397-100UG Plastic ampoule 100 μg
569397-1MG Plastic ampoule 1 mg
569397-250UG Plastic ampoule 250 μg
Description
OverviewA potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases. Inhibits protein kinase A (IC50 = 7 nM), CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase C (IC50 = 700 pM), and protein kinase G (IC50 = 8.5 nM). Also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation. Induces apoptosis in human malignant glioma cell lines. Arrests normal cells at the G1 checkpoint. A 1 mM (100 µg/214 µl) solution of Staurosporine (Cat. No. 569396) in DMSO is also available.
Catalogue Number569397
Brand Family Calbiochem®
SynonymsMLCK Inhibitor I, PKA Inhibitor II
References
ReferencesCouldwell, W.T., et al. 1994. FEBS Lett. 345, 43.
Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.
Bruno, S., et al. 1992. Cancer Res. 52, 470.
Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.
Vitale, M.L., et al. 1992. Neuroscience 51, 463.
Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol. 28, 102.
Oka, S., et al. 1986. Agric. Biol. Chem. 50, 2723.
Product Information
CAS number62996-74-1
ATP CompetitiveY
FormWhite to pale yellow solid
Hill FormulaC₂₈H₂₆N₄O₃
Chemical formulaC₂₈H₂₆N₄O₃
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetPKA
Primary Target IC<sub>50</sub>7 nM, 20 nM, 1.3 nM, 700 pM, and 8.5 nM against protein kinase A , CaM kinase, myosin light chain kinase, protein kinase C, and protein kinase G, respectively
Purity≥99% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
569397-100UG 07790788057855
569397-1MG 04055977190465
569397-250UG 04055977190496

Documentation

Staurosporine, Streptomyces sp. SDS

Title

Safety Data Sheet (SDS) 

Staurosporine, Streptomyces sp. Certificates of Analysis

TitleLot Number
569397

References

Reference overview
Couldwell, W.T., et al. 1994. FEBS Lett. 345, 43.
Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.
Bruno, S., et al. 1992. Cancer Res. 52, 470.
Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.
Vitale, M.L., et al. 1992. Neuroscience 51, 463.
Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol. 28, 102.
Oka, S., et al. 1986. Agric. Biol. Chem. 50, 2723.

Brochure

Title
PKC Pathway Poster PDF ( 676 KB )

Citations

Title
  • Joao B. Oliveira, et al. (2007) NRAS mutation causes a human autoimmune lymphoproliferative syndrome. Procedings of the National Academy of Science 104, 8953-8958.
  • Siva Kumar Kolluri, et al. (2005) The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis. Procedings of the National Academy of Science 102, 2525-2530.
  • Rick B. Vega, et al. (2004) Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5. Molecular and Cellular Biology 24, 8374-8385.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-July-2014 JSW
    SynonymsMLCK Inhibitor I, PKA Inhibitor II
    DescriptionA potent, cell-permeable, reversible, ATP-competitive and broad spectrum inhibitor of protein kinases. Inhibits CaM kinase (IC50 = 20 nM), myosin light chain kinase (IC50 = 1.3 nM), protein kinase A (IC50 = 7 nM), protein kinase C (IC50 = 0.7 nM), and protein kinase G (IC50 = 8.5 nM). Also inhibits platelet aggregation induced by collagen or ADP but has no effect on thrombin-induced platelet aggregation. Induces apoptosis in human malignant glioma cell lines. Arrests normal cells at the G1 checkpoint.
    FormWhite to pale yellow solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number62996-74-1
    Chemical formulaC₂₈H₂₆N₄O₃
    Structure formulaStructure formula
    Purity≥99% by HPLC
    SolubilityDMSO (5 mg/ml). Further dilute with aqueous buffers just prior to use.
    Storage Protect from light
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
    Toxicity Standard Handling
    ReferencesCouldwell, W.T., et al. 1994. FEBS Lett. 345, 43.
    Nishimura, H. and Simpson, I.A. 1994. Biochem. J. 302, 271.
    Bruno, S., et al. 1992. Cancer Res. 52, 470.
    Kiss, Z. and Deli, E. 1992. Biochem. J. 288, 853.
    Vitale, M.L., et al. 1992. Neuroscience 51, 463.
    Hoffman, R. and Newland, E.S. 1991. Cancer Chemother. Pharmacol. 28, 102.
    Oka, S., et al. 1986. Agric. Biol. Chem. 50, 2723.
    Citation
  • Joao B. Oliveira, et al. (2007) NRAS mutation causes a human autoimmune lymphoproliferative syndrome. Procedings of the National Academy of Science 104, 8953-8958.
  • Siva Kumar Kolluri, et al. (2005) The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis. Procedings of the National Academy of Science 102, 2525-2530.
  • Rick B. Vega, et al. (2004) Protein Kinases C and D Mediate Agonist-Dependent Cardiac Hypertrophy through Nuclear Export of Histone Deacetylase 5. Molecular and Cellular Biology 24, 8374-8385.
    		•

    Related Products & Applications

    Categories

    Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Protein Kinase C (PKC) Inhibitors
    Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Protein Kinase A (PKA; cAMP-Dependent Protein Kinase) Inhibitors
    Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > Protein Kinase G (PKG; cGMP-Dependent Protein Kinase) Inhibitors