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Steritop™ Filter Units

Steritop bottle top filter units provide a large filter area for rapid sterile filtration of aqueous fluids into narrow-mouth (33 mm) or wide-mouth (45 mm) bottles.

Steritop bottle top filter units provide a large filter area for rapid sterile filtration of aqueous fluids into narrow-mouth (33 mm) or wide-mouth (45 mm) bottles. A small tab inside the funnel secures an optional prefilter in-place during filtration. For added protection against contamination, the threads for bottle attachment have been slightly recessed in case the funnel accidentally touches a work surface.

Overview

Specifications

Ordering Information

Units with fast flow and low protein binding 0.22 µm Millipore Express PLUS (PES) membrane for sterilization of aqueous solutions*Clear Sorting & Filtering Show Filter
Catalog NumberDescriptionFunnel CapacityFitting OutletPack Size
SCGPS01RESteritop Threaded Bottle Top Filter 150 mL 33 mm thread 12 pack, GP 0.22 µm Polyethersulfone 150 mL 33 mm Radio-sterilized
SCGPS02RESteritop Threaded Bottle Top Filter 250 mL 33 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 250 mL 33 mm, radio-sterilized
SCGPS05RESteritop Threaded Bottle Top Filter 500 mL 33 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 500 mL 33 mm, radio-sterilized

* These products have been tested and validated for use in stem cell research applications. To determine their effects on Mouse Stem Cell growth or differentiation, three lots of Steritop-GP devices were used to filter media with LIF. Once filtered, this media was used to passage mouse stem cells five times to verify that Steritop-GP filtration does not impact pluripotency of mouse stem cells.

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Documentation

FAQ

QuestionAnswer
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Why use a vacuum driven filter rather than a pressure driven filter?Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
Should I filter my acrylamide solution prior to gel casting?Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
2. It will aid in degassing, which will promote efficient polymerization of the gel.

Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
Which pre-filter should I be using with my Stericup or Steritop unit?You should use an AP20 75mm pre-filter (AP2007500). To help keep the filter from floating in the solution to be filtered, insert the pre-filter under the pre-filter tab inside the Stericup or Steritop.
What is the expiration date on Stericup and Steritop units?The expiration date, which specifies product sterility, for the Stericup and Steritop products is three years from the date of manufacture.
What type of flow rates can I expect with the Stericups and Steritops?For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm.
Are Stericup/tops compatible with formamide?Yes. Formamide is probably the least organic compound. It's almost like water. So it would be compatible with everything. The potential mistake is mistaking formamide for dimethylformamide, which is a powerful organic solvent.
Are Stericup and Steritop units autoclavable?No. The highest temperature that the product can be used at is 50C ( 122F ).
Can I bubble point my Stericup/top?No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.

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Categories

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices