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69522 T7•Tag® Monoclonal Antibody

The T7•Tag antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors.

T7•Tag® Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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69522
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
69522-3 Plastic ampoule 50 μg
69522-4 Plastic ampoule 250 μg
Description
OverviewThe T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/IsotypeMouse monoclonal IgG2b
Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
FormStabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution1:5,000–1:10,000
Catalogue Number69522
Brand Family Novagen®
References
References1. Krek, W., et al. 1994. Cell 78,, 161.

2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
69522-3 04055977274448
69522-4 07790788052584

Documentation

T7•Tag® Monoclonal Antibody Certificates of Analysis

TitleLot Number
69522

References

Reference overview
1. Krek, W., et al. 1994. Cell 78,, 161.

2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.

Brochure

Title
Bulk packaging
Protein Purification and Detection Tools

Citations

Title
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    • User Protocols

    Title
    TB015 T7•Tag® Antibody